Huang Luyao, Li Ziyu, Lou Yuntian, Cao Fahe, Zhang Dawei, Li Xiaogang
Institute for Advanced Materials and Technology, University of Science and Technology Beijing, Beijing 100083, China.
Department of Chemistry, Zhejiang University, Hangzhou 310027, China.
Materials (Basel). 2018 Aug 9;11(8):1389. doi: 10.3390/ma11081389.
Scanning electrochemical microscopy (SECM) is a chemical microscopy technique with high spatial resolution for imaging sample topography and mapping specific chemical species in liquid environments. With the development of smaller, more sensitive ultramicroelectrodes (UMEs) and more precise computer-controlled measurements, SECM has been widely used to study biological systems over the past three decades. Recent methodological breakthroughs have popularized SECM as a tool for investigating molecular-level chemical reactions. The most common applications include monitoring and analyzing the biological processes associated with enzymatic activity and DNA, and the physiological activity of living cells and other microorganisms. The present article first introduces the basic principles of SECM, followed by an updated review of the applications of SECM in biological studies on enzymes, DNA, proteins, and living cells. Particularly, the potential of SECM for investigating bacterial and biofilm activities is discussed.
扫描电化学显微镜（SECM）是一种化学显微镜技术，具有高空间分辨率，可用于在液体环境中对样品形貌进行成像以及绘制特定化学物质的分布图。随着更小、更灵敏的超微电极（UME）的发展以及更精确的计算机控制测量技术的出现，在过去三十年中，SECM已被广泛用于研究生物系统。最近的方法学突破使SECM作为一种研究分子水平化学反应的工具得到了普及。最常见的应用包括监测和分析与酶活性和DNA相关的生物过程，以及活细胞和其他微生物的生理活性。本文首先介绍SECM的基本原理，随后对SECM在酶、DNA、蛋白质和活细胞生物学研究中的应用进行最新综述。特别讨论了SECM在研究细菌和生物膜活性方面的潜力。
