Cho W-S, Chae C
Department of Veterinary Pathology, College of Veterinary Medicine, Seoul National University, Kwanak-Gu, Republic of Korea.
Vet Pathol. 2004 Jul;41(4):419-23. doi: 10.1354/vp.41-4-419.
Cyclooxygenase-2 (COX-2) and nitric oxide synthase 2 (NOS2) were detected and localized in 20 pigs with ulcerative colitis caused by natural infection with Salmonella typhimurium. Evidence of NOS2 activity was determined by the formation of nitrotyrosine, a reaction product of peroxynitrite, in NOS2-expressing ulcerative colons by immunohistochemistry. Transcript RNA of COX-2 and NOS2 was consistently detected in colonic tissues from the 20 pigs with ulcerative colitis by using reverse transcription-polymerase chain reaction. Immunohistochemical signals for COX-2 and NOS2 were detected in the ulcerated area of all 20 pigs. Expression of COX-2 and NOS2 was identified continuously within inflammatory intestinal lesions but was minimal in unaffected regions of the colon of S. typhimurium-infected pigs. The immunohistochemistry of serial sections of intestine indicated that the majority of colons containing numerous COX-2-positive cells also had numerous NOS2-positive cells. Localization of NOS2 and a nitrotyrosine antigen was prominent in neutrophils and macrophages in the periphery of the lesions. Simultaneous detection of COX-2 and NOS2 RNA and protein indicated functional activity of prostaglandin and NO production in vivo. This study suggested that COX-2 and NOS2 expression may play a role in the pathophysiologic processes in ulcerative colitis caused by S. typhimurium.
在20头因自然感染鼠伤寒沙门氏菌而患溃疡性结肠炎的猪中，检测了环氧化酶-2（COX-2）和一氧化氮合酶2（NOS2）并确定了其定位。通过免疫组织化学方法，在表达NOS2的溃疡性结肠中，通过过氧亚硝酸盐的反应产物硝基酪氨酸的形成来确定NOS2活性的证据。使用逆转录-聚合酶链反应，在20头患有溃疡性结肠炎的猪的结肠组织中持续检测到COX-2和NOS2的转录RNA。在所有20头猪的溃疡区域均检测到COX-2和NOS2的免疫组织化学信号。COX-2和NOS2的表达在炎症性肠病变中持续存在，但在鼠伤寒沙门氏菌感染猪的结肠未受影响区域中最低。肠道连续切片的免疫组织化学表明，大多数含有大量COX-2阳性细胞的结肠也有大量NOS2阳性细胞。NOS2和硝基酪氨酸抗原在病变周边的中性粒细胞和巨噬细胞中定位明显。COX-2和NOS2 RNA及蛋白质的同时检测表明体内前列腺素和一氧化氮产生具有功能活性。本研究提示COX-2和NOS2表达可能在鼠伤寒沙门氏菌引起的溃疡性结肠炎的病理生理过程中起作用。
