一种用于检测过量用药后人血浆中羟嗪的简单高效液相色谱法。
A simple high-performance liquid chromatographic method for detection of hydroxyzine in human plasma after overdose.
作者信息
Péhourcq Fabienne
机构信息
Department of Clinical Pharmacology and Toxicology, CHU Pellegrin, Place Amélie Raba-Léon, 33076 Bordeaux Cedex, France.
出版信息
J Pharmacol Toxicol Methods. 2004 Jul-Aug;50(1):41-4. doi: 10.1016/j.vascn.2004.01.001.
INTRODUCTION
Hydroxyzine, a piperazine H1-receptor antagonist, is effective in generalised anxiety disorder. For toxicological purposes, a simple reversed-phase high-performance liquid chromatographic assay was developed for the detection of hydroxyzine in human plasma.
METHODS
A liquid-liquid procedure was used to extract the drug from plasma in the presence of an internal standard (clothiapine). The analysis was performed on a Spherisorb S5 C(8) analytical column with UV detection.
RESULTS
A linear response was observed over the concentration range 20-1500 ng/ml. A good accuracy (bias<7.3%) was achieved for all quality controls, with intraday and interday variation coefficients inferior to 8.5%. The limit of detection was 10 ng/ml, without interference of endogenous components.
DISCUSSION
This rapid method (run time<13 min) is currently used for poison management involving hydroxyzine.
引言
羟嗪是一种哌嗪类H1受体拮抗剂，对广泛性焦虑症有效。出于毒理学目的，开发了一种简单的反相高效液相色谱法用于检测人血浆中的羟嗪。
方法
采用液-液萃取法在存在内标（氯氮平）的情况下从血浆中提取药物。分析在Spherisorb S5 C(8)分析柱上进行，采用紫外检测。
结果
在20 - 1500 ng/ml的浓度范围内观察到线性响应。所有质量控制的准确度良好（偏差<7.3%），日内和日间变异系数均低于8.5%。检测限为10 ng/ml，无内源性成分干扰。
讨论
这种快速方法（运行时间<13分钟）目前用于涉及羟嗪的中毒处理。
Zotero 插件