Wang Tzu-Hao, Lee Yun-Shien, Chen En-Shih, Kong Wei-Hsiang, Chen Lung-Kun, Hsueh Ding-Wei, Wei Min-Li, Wang Hsing-Shih, Lee Ying-Shiung
Department of Obstetrics and Gynecology, Chang Gung Memorial Hospital, Tao-Yuan, Taiwan, ROC.
Chang Gung Med J. 2004 Apr;27(4):243-60.
Advances in molecular and computational biology have led to the development of powerful, high-throughput methods for analysis of differential gene expression, which are opening up new opportunities in genomic medicine. DNA microarray technology has been enthusiastically integrated into basic biomedical research and will eventually become a molecular monitoring tool for various clinical courses.
As a core research facility of Chang Gung University (CGU) and Chang Gung Memorial Hospital (CGMH), the Genomic Medicine Research Core Laboratory (GMRCL) welcomes investigators from every discipline to employ DNA microarray technology in the quest for knowledge of genomic medicine. The first tasks for GMRCL are to optimize the standard operating procedures (SOP) for each instrument and to assure the quality of every procedure.
During the first year after the establishment of the GMRCL at the CGMH, we tested and adopted procedures that were satisfactory for our purposes. These procedures included: replication of bacterial stocks, amplification of human DNA clones, annotation of each DNA clone, production of cDNA microarrays, validation of RNA quality and quantity, labeling of target specimens, competitive hybridization, scanning of slides, data analysis, and post-microarray validation of results. We present a summarization of the materials and procedures used at the GMRCL and discuss the reasons for using them.
The information about the cDNA microarray analysis system at the GMRCL is compliant with the minimal information about a microarray experiment (MIAME) format. The information may be useful to both the investigators who are using this core facility and researchers at other institutes, who will establish their own in-house cDNA microarray systems.
分子生物学和计算生物学的进展促使了强大的高通量差异基因表达分析方法的发展，这些方法为基因组医学带来了新机遇。DNA微阵列技术已被积极应用于基础生物医学研究，并最终将成为各种临床病程的分子监测工具。
作为长庚大学（CGU）和长庚纪念医院（CGMH）的核心研究机构，基因组医学研究核心实验室（GMRCL）欢迎各学科的研究人员运用DNA微阵列技术探索基因组医学知识。GMRCL的首要任务是优化各仪器的标准操作程序（SOP）并确保每个程序的质量。
在CGMH的GMRCL成立后的第一年，我们测试并采用了符合我们需求的程序。这些程序包括：细菌菌株的复制、人类DNA克隆的扩增、每个DNA克隆的注释、cDNA微阵列的制备、RNA质量和数量的验证、靶标本的标记、竞争性杂交、玻片扫描、数据分析以及微阵列结果的后期验证。我们总结了GMRCL所使用的材料和程序，并讨论了使用它们的原因。
GMRCL的cDNA微阵列分析系统的信息符合微阵列实验最小信息（MIAME）格式。这些信息对于使用该核心设施的研究人员以及其他将建立自己内部cDNA微阵列系统的研究所的研究人员可能有用。
