Guzman J, Wang Y M, Kalaycioglu O, Schoenfeld B, Hamm H, Bartsch W, Costabel U
Department of Pathology, Ruhr University, Bochum, Germany.
Acta Cytol. 1992 Sep-Oct;36(5):668-73.
Surfactant protein A (SP-A) appears to have an important function in the assembly and maintenance of the alveolar surfactant monolayer. SP-A has also been implicated in modulating the activity of immunoactive cells, such as increasing the bactericidal capacity of alveolar macrophages. In this immunocytochemical study the SP-A content of alveolar macrophages from seven patients with hypersensitivity pneumonitis was compared with the results obtained from six healthy controls. A polyclonal rabbit antibody against human SP-A was used for detection of SP-A in the cytoplasm of alveolar macrophages, applying the immunoperoxidase adhesive slide assay. In hypersensitivity pneumonitis a significant increase in the percentage of SP-A+ alveolar macrophages was observed as compared with the percentage in healthy controls. The intensity of the staining reaction was also increased in the alveolar macrophages of hypersensitivity pneumonitis. We conclude that the observed abnormalities in SP-A content in alveolar macrophages may play a role in the pathogenesis of hypersensitivity pneumonitis.
表面活性蛋白A（SP-A）似乎在肺泡表面活性物质单分子层的组装和维持中具有重要作用。SP-A还与调节免疫活性细胞的活性有关，例如提高肺泡巨噬细胞的杀菌能力。在这项免疫细胞化学研究中，将7例过敏性肺炎患者肺泡巨噬细胞中的SP-A含量与6例健康对照者的结果进行了比较。使用针对人SP-A的多克隆兔抗体，通过免疫过氧化物酶黏附玻片测定法检测肺泡巨噬细胞胞质中的SP-A。与健康对照者相比，过敏性肺炎患者中SP-A阳性肺泡巨噬细胞的百分比显著增加。过敏性肺炎患者肺泡巨噬细胞中的染色反应强度也有所增加。我们得出结论，观察到的肺泡巨噬细胞中SP-A含量异常可能在过敏性肺炎的发病机制中起作用。
