Yamamoto Tetsuji, Marui Takashi, Akisue Toshihiro, Nakatani Tetsuya, Fujita Ikuo, Matsumoto Keiji, Hitora Toshiaki, Kawamoto Teruya, Nagira Keiko, Kurosaka Masahiro
Department of Orthopedic Surgery, Kobe University, Graduate School of Medicine, 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650-0017, Japan.
Pathol Res Pract. 2004;200(5):397-402. doi: 10.1016/j.prp.2004.03.007.
Hepatocyte growth factor (HGF) is a multifunctional cytokine that variably affects cell motility, proliferation, and morphogenesis. Little information is currently available on the HGF and its receptor c-Met expression in malignant fibrous histiocytoma (MFH). We immunohistochemically investigated the HGF and c-Met expression in 43 MFH tissue specimens. Furthermore, the correlation of the HGF and c-Met expression with tumor proliferative activity assessed by MIB-1 indices was analyzed. Our results showed that positive cytoplasmic immunoreactivity for HGF and c-Met was identified in tumor cells in 36 (84%) and 20 (47%) of the 43 MFH cases analyzed, respectively. Coexpression of HGF and c-Met was observed in 20 (47%) of the 43 MFHs, and was correlated with high MIB-1 proliferative indices (p = 0.0446). These findings strongly indicate that the HGF/c-Met signaling system plays an important role in promoting cell proliferation of human MFHs via an autocrine loop.
肝细胞生长因子（HGF）是一种多功能细胞因子，可不同程度地影响细胞运动、增殖和形态发生。目前关于恶性纤维组织细胞瘤（MFH）中HGF及其受体c-Met表达的信息较少。我们采用免疫组织化学方法研究了43例MFH组织标本中HGF和c-Met的表达情况。此外，还分析了HGF和c-Met表达与通过MIB-1指数评估的肿瘤增殖活性之间的相关性。我们的结果显示，在分析的43例MFH病例中，分别有36例（84%）和20例（47%）的肿瘤细胞中检测到HGF和c-Met的细胞质免疫反应阳性。在43例MFH中有20例（47%）观察到HGF和c-Met的共表达，且与高MIB-1增殖指数相关（p = 0.0446）。这些发现有力地表明，HGF/c-Met信号系统通过自分泌环在促进人MFH细胞增殖中起重要作用。
