Cheng Tzu-Chun, Huang Chun-Chia, Huang Lii-Sheng, Chen Chung-I, Lee Maw-Sheng, Liu Jer-Yuh
Institute of Biochemistry, Medical College, Chung-Shan Medical University, Taichung, Taiwan, ROC.
Chin J Physiol. 2004 Mar 31;47(1):43-7.
The aim of our study is to observe the relationship between the blastocyst morphology and the implantation rate for mice. Mouse embryos obtained from the superovulated-ICR mice were cultured in vitro from 1-cell zygotes to blastocysts. Mouse blastocysts were then classified into 3 grades: grade I, small blastocysts; grade II, large blastocysts; grade III, hatching blastocysts. They were independently transferred into the uterus of recipient females mated with vasectomized male mice on 96 hours after the zygotes were cultured in vitro. The successful implantation was checked by injection of Chicago Sky Blue 6B on the second day after embryo transfer. Although there was no significant difference in the implantation rates between the grade III and grade II, grade I was significantly decreased, as compared with grade III. Grade I and grade II was also significantly decreased in both the diameter of blastocysts and cell number of inner cell mass (ICM) and trophectoderm (TE), as compared with grade III. These findings indicate that the expanded and hatching blastocyst selections for embryo transfer in in vitro fertilization were evaluated with the high implantation rate.
我们研究的目的是观察小鼠囊胚形态与着床率之间的关系。从超排卵的ICR小鼠获得的小鼠胚胎从1细胞合子开始进行体外培养直至囊胚。然后将小鼠囊胚分为3个等级：I级，小囊胚；II级，大囊胚；III级，孵化囊胚。在合子体外培养96小时后，将它们分别移植到与输精管结扎的雄性小鼠交配的受体雌性小鼠的子宫中。在胚胎移植后的第二天，通过注射芝加哥天蓝6B来检查是否成功着床。尽管III级和II级的着床率没有显著差异，但与III级相比，I级的着床率显著降低。与III级相比，I级和II级在囊胚直径、内细胞团（ICM）和滋养外胚层（TE）的细胞数量方面也显著降低。这些发现表明，在体外受精中，选择扩张和孵化的囊胚进行胚胎移植时，着床率较高。
