Nili H, Mesbah F, Kafi M, Nasr Esfahani M H
Department of Clinical Science, School of Veterinary Medicine, Shiraz University 71345, PO Box 1731, Shiraz, Iran.
Anat Histol Embryol. 2004 Aug;33(4):196-9. doi: 10.1111/j.1439-0264.2004.00536.x.
In order to provide a consistent system for laboratory production of embryos, the characteristics of immature camel oocyte must first be described. The objective of this study was to define ultrastructural features of immature camel oocyte. Ovaries were obtained from camels at a local abattoir, and then transported to the laboratory within 2 h. Camelus cumulus oocyte complexes (COCs) were aspirated from 2-6 mm follicles using a 22-gauge needle. Excellent and good quality COCs were selected and prepared for transmission electron microscopy study using a cavity slide. The fine structure of camel oocyte is morphologically similar to that of other mammalian oocytes. However, some minor differences exist between COC of camel and other mammalian species. Different size and shape of membrane-bound vesicles, lipid droplet, mitochondria and cortical granules were distributed throughout the ooplasm. Discrete or in association with endoplasmic reticulum, Golgi complexes were observed in the periphery of the oocytes. The majority of the oocytes were in the germinal vesicle stage.
为了建立一个用于实验室胚胎生产的一致系统，必须首先描述未成熟骆驼卵母细胞的特征。本研究的目的是确定未成熟骆驼卵母细胞的超微结构特征。卵巢取自当地屠宰场的骆驼，然后在2小时内运至实验室。使用22号针头从2 - 6毫米的卵泡中吸出骆驼卵丘卵母细胞复合体（COCs）。选择优质和良好质量的COCs，使用腔室载玻片制备用于透射电子显微镜研究。骆驼卵母细胞的精细结构在形态上与其他哺乳动物卵母细胞相似。然而，骆驼的COC与其他哺乳动物物种之间存在一些细微差异。不同大小和形状的膜结合囊泡、脂滴、线粒体和皮质颗粒分布于整个卵质中。在卵母细胞周边观察到离散的或与内质网相关的高尔基体。大多数卵母细胞处于生发泡期。
