通过显微切割技术靶向生成牛染色体5q21-q25区域的16个序列标签位点。

Targeted generation of 16 sequence-tagged sites for bovine chromosome region 5q21-q25 by microdissection.

作者信息

Goldammer Tom, Rottengatter Karin, Weikard Rosemarie, Horstmann Rolf, Gehlhaus Annette, Brunner Ronald M, Hanotte Olivier, Schwerin Manfred

机构信息

Forschungsbereich Molekularbiologie, Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere (FBN), Wilhelm-Stahl-Allee 2, D-18196 Dummerstorf, Germany.

出版信息

Chromosome Res. 2004;12(4):309-15. doi: 10.1023/B:CHRO.0000034102.54635.b9.

DOI:10.1023/B:CHRO.0000034102.54635.b9

PMID:15241011

Abstract

The cattle chromosome region BTA5q21-q25 is associated with immune defense and is linked with several economically important traits for growth, ovulation, twinning and milk yield. Chromosome microdissection of the region was performed to generate fragment-specific DNA libraries and to provide new bovine-specific DNA sequences as starting material for physical fine-mapping approaches or linkage studies. A first library screening resulted in the identification of 16 region-specific sequence-tagged sites (STS). Fifteen STS represent new unique cattle DNA sequences, of which one identifies a new microsatellite. Homology with the gene CDK2 was found for the remaining STS.

摘要

牛染色体区域BTA5q21 - q25与免疫防御相关，并且与生长、排卵、产双胎和产奶量等几个重要经济性状相关联。对该区域进行染色体显微切割以生成片段特异性DNA文库，并提供新的牛特异性DNA序列作为物理精细定位方法或连锁研究的起始材料。首次文库筛选鉴定出16个区域特异性序列标签位点（STS）。其中15个STS代表新的独特牛DNA序列，其中一个鉴定出一个新的微卫星。其余的STS与CDK2基因存在同源性。