Wang S X, Sng L H, Leong H N, Tan B H
Central Tuberculosis Laboratory, Department of Pathology, Singapore General Hospital, Outram Rd., Singapore 169608.
J Clin Microbiol. 2004 Jul;42(7):3336-8. doi: 10.1128/JCM.42.7.3336-3338.2004.
PCR-restriction endonuclease analysis (PRA) was used for direct identification of Mycobacterium haemophilum in clinical specimens from immunocompromised patients. PRA correctly identified M. haemophilum in four smear-positive specimens. Direct identification by PRA takes 2 to 3 working days compared to the 3 to 5 weeks required for culture isolation and identification by conventional methods.