CEPH酵母人工染色体文库

The CEPH YAC library.

作者信息

Dausset J, Ougen P, Abderrahim H, Billault A, Sambucy J L, Cohen D, Le Paslier D

机构信息

Centre d'Etude du Polymorphisme Humain (C.E.P.H.), Paris.

出版信息

Behring Inst Mitt. 1992 Apr(91):13-20.

PMID:1524562

Abstract

Because of their large size, YACs provide is a powerful tool for physical mapping studies of complex genomes. As it will be advantageous to have genomic libraries of clones with large inserts for analyzing megabase sized regions of the human genome, we have investigated a number of parameters in order to increase the insert size of the YACs. We constructed a genomic library currently containing more than 85,000 YAC clones. Mean sizes of YACs produced at several stages of construction of the library range from 430 kb to 1,200 kb, representing 13 haploid equivalents of the human genome. This library was organized in order to allow rapid screen of YACs for large scale physical mapping of the human genome.

摘要

由于酵母人工染色体（YACs）尺寸较大，它们为复杂基因组的物理图谱研究提供了一个强大的工具。鉴于拥有大插入片段克隆的基因组文库对于分析人类基因组的兆碱基大小区域将是有利的，我们研究了许多参数以增加YACs的插入片段大小。我们构建了一个目前包含超过85,000个YAC克隆的基因组文库。在文库构建的几个阶段产生的YACs的平均大小范围从430 kb到1,200 kb，代表了人类基因组的13个单倍体当量。该文库经过整理，以便能够快速筛选YACs用于人类基因组的大规模物理图谱绘制。