[用于通过聚合酶链反应合成RNA的具有特定结构的DNA基质的设计]
[Design of a DNA-matrix with a specific structure for synthesizing RNA using the polymerase chain reaction].
作者信息
Smelkova N V, Elov A A, Shabarova Z A
出版信息
Bioorg Khim. 1992 Jan;18(1):78-84.
PMID:1524586
Abstract
Combination of chemical DNA synthesis and polymerase chain reactions has been used for obtaining DNA templates of preset structure coding for target mRNAs. The obtained DNA were 136 to 246 bp in length, contained promoters for RNA polymerases of phage T7 or E. coli and coded for 136-membered mRNA.
摘要
化学DNA合成与聚合酶链反应相结合已被用于获得编码靶mRNA的预设结构的DNA模板。所获得的DNA长度为136至246个碱基对，包含噬菌体T7或大肠杆菌RNA聚合酶的启动子，并编码136个成员的mRNA。
Zotero 插件