Denisova L Ia, Zagrebel'nyy S N, Pustoshilova N M, Ven'iaminova A G, Gorn V V
Bioorg Khim. 1989 Apr;15(4):562-5.
A new approach to synthesis of oligoribonucleotides is suggested, based on transcription by E. coli RNA polymerase of synthetic immobilized DNA-templates with AUG as primer. The approach has been experimentally verified by synthesis of two oligonucleotides, viz., a RNA fragment of the fr phage (16 nucleotides long) and a RNA fragment of the tickborne encephalitis virus (18 nucleotides long). Fraction of the synthesized RNA fragments in the whole nucleotide material is about 20%. The templates can be used repeatedly. Sequences of the oligoribonucleotides were confirmed. Advantages of this approach and its usefulness for SP6 DNA-dependent RNA polymerase are discussed.
提出了一种合成寡核糖核苷酸的新方法，该方法基于大肠杆菌RNA聚合酶以AUG为引物对合成的固定化DNA模板进行转录。通过合成两种寡核苷酸，即噬菌体fr的RNA片段（16个核苷酸长）和蜱传脑炎病毒的RNA片段（18个核苷酸长），对该方法进行了实验验证。合成的RNA片段在整个核苷酸材料中的比例约为20%。这些模板可以重复使用。寡核糖核苷酸的序列得到了确认。讨论了该方法的优点及其对SP6 DNA依赖性RNA聚合酶的实用性。
