Vanitha J Daisy, Paramasivan C N
Tuberculosis Research Centre, Chennai 600 031, India.
Diagn Microbiol Infect Dis. 2004 Jul;49(3):179-82. doi: 10.1016/j.diagmicrobio.2004.04.003.
Fifty-one clinical isolates and 5 clarithromycin-resistant mutants of Mycobacterium avium complex (MAC) were tested for their susceptibility to clarithromycin by microplate Alamar blue assay (MABA). The susceptibility results were compared with the results obtained by the BACTEC 460 method. All clinical isolates were susceptible, while all mutants were resistant to clarithromycin by BACTEC. Eighty-six percent of the clinical isolates were susceptible by MABA, and one of the resistant mutants was misclassified as susceptible by this method. The overall agreement between MABA and BACTEC was 86%, indicating the usefulness of MABA in drug susceptibility testing of MAC.
采用微孔板阿拉玛蓝法（MABA）对51株鸟分枝杆菌复合群（MAC）临床分离株和5株克拉霉素耐药突变株进行克拉霉素敏感性检测。将药敏结果与BACTEC 460法所得结果进行比较。所有临床分离株对克拉霉素敏感，而通过BACTEC法检测所有突变株均对克拉霉素耐药。通过MABA法检测，86%的临床分离株敏感，且有1株耐药突变株被该方法误判为敏感。MABA法与BACTEC法的总体一致性为86%，表明MABA法在MAC药敏试验中具有实用性。
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