Division of Medical Microbiology, Department of Pathology, Johns Hopkins University, Baltimore, MD 21287, USA.
Diagn Microbiol Infect Dis. 2010 May;67(1):47-51. doi: 10.1016/j.diagmicrobio.2009.12.008. Epub 2010 Mar 12.
Standard susceptibility testing of the Mycobacterium avium complex (MAC) can require 7 to 14 days from initial isolation. We evaluated a high-performance liquid chromatography-based susceptibility test for rapid determination of clarithromycin (CLR) resistance in MAC. This method can be completed in 72 h of incubation. A total of 110 MAC strains were tested using the following concentrations of CLR: 4, 16, and 64 microg/mL, for a total of 330 tests. Microbroth dilution was used as the reference method. Rapid analysis of mycolic acid ("RAM") concordance with the reference method for CLR susceptibility was 98% (254/258) and 100% for CLR resistance (72/72). The 4 discordant results occurred with 2 strains, which demonstrated intermediate resistance with an MIC of 16 microg/mL. This study demonstrates that "RAM"-based susceptibility testing for determination of CLR resistance in MAC is both rapid and accurate, providing a significant reduction in turn-around-time from 7 to 14 days to 72 h of incubation.
对鸟分枝杆菌复合群（MAC）进行标准药敏试验，从初始分离到获得结果通常需要 7 至 14 天。我们评估了一种基于高效液相色谱的药敏试验方法，用于快速检测 MAC 对克拉霉素（CLR）的耐药性。这种方法的孵育时间总计为 72 小时。使用以下 CLR 浓度对总共 110 株 MAC 菌株进行了测试：4、16 和 64μg/mL，共进行了 330 次测试。微量肉汤稀释法作为参考方法。快速分析分枝菌酸（"RAM"）与 CLR 药敏参考方法的一致性为 98%（254/258），对 CLR 耐药性的一致性为 100%（72/72）。4 次不一致的结果发生在 2 株菌株上，这两株菌株的 MIC 为 16μg/mL，表现出中介耐药性。本研究表明，基于 RAM 的药敏试验用于检测 MAC 中 CLR 的耐药性，既快速又准确，将从 7 至 14 天的周转时间缩短到 72 小时的孵育时间。
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