α5 GABAA 受体亚基在发育中大鼠海马体中的表达。
Expression of alpha 5 GABAA receptor subunit in developing rat hippocampus.
作者信息
Ramos Blanca, Lopez-Tellez Juan Felix, Vela Jose, Baglietto-Vargas David, del Rio Juan Carlos, Ruano Diego, Gutierrez Antonia, Vitorica Javier
机构信息
Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/Prof. Garcia Gonzalez, 41012, Spain.
出版信息
Brain Res Dev Brain Res. 2004 Jul 19;151(1-2):87-98. doi: 10.1016/j.devbrainres.2004.04.003.
The GABAergic system plays an important role in the hippocampal development. Here we have studied the developmental expression of the alpha 5 subunit of the GABA(A) receptor (from rat hippocampus) by RT-competitive PCR, immunoblot and immunocytochemistry. Our results demonstrated an early induction of the alpha 5 subunit expression (at mRNA and protein levels) during the first postnatal week, peaking at P5 and decreasing after this age. The peak of alpha 5 subunit expression precedes the peak of expression for the synaptophysin, GAD65 and GAD67. Thus, the increase in the alpha 5 GABA(A) receptor subunit expression may precede the GABAergic synaptogenesis. Importantly, between P0 and P7, the expression of the alpha 5 subunit was concentrated at the cell somata of the pyramidal and granular cells. After P10, its localization shifted from the cell bodies to the dendritic layers. This developmental pattern is similar to that reported for the Na(+)-K(+)-2Cl(-) system and it might be correlated with the transition from excitatory to inhibitory GABAergic activity.
γ-氨基丁酸（GABA）能系统在海马体发育中起重要作用。在此，我们通过逆转录竞争聚合酶链反应（RT-competitive PCR）、免疫印迹和免疫细胞化学方法研究了GABA(A)受体α5亚基（来自大鼠海马体）的发育表达情况。我们的结果表明，在出生后的第一周，α5亚基表达（在mRNA和蛋白质水平）出现早期诱导，在出生后第5天达到峰值，并在此年龄之后下降。α5亚基表达的峰值先于突触素、谷氨酸脱羧酶65（GAD65）和谷氨酸脱羧酶67（GAD67）表达的峰值。因此，α5 GABA(A)受体亚基表达的增加可能先于GABA能突触形成。重要的是，在出生后第0天至第7天之间，α5亚基的表达集中在锥体细胞和颗粒细胞的细胞体上。出生后第10天之后，其定位从细胞体转移到树突层。这种发育模式与报道的钠-钾-2氯协同转运体（Na(+)-K(+)-2Cl(-)）系统相似，并且可能与从兴奋性GABA能活动向抑制性GABA能活动的转变相关。
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