Pandey Ratna, Deshpande Shripad B
Department of Physiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi 221005, India.
Toxicon. 2004 Aug;44(2):201-5. doi: 10.1016/j.toxicon.2004.05.025.
Role of aprotinin (kallikrein-kinin synthesis inhibitor) in preventing the cardio-respiratory toxicity induced by Mesobuthus tamulus (BT) venom was evaluated. The effects of BT venom (5 mg/kg) on mean arterial pressure (MAP), heart rate (HR), respiratory rate (RR), lung compliance and pulmonary water content were examined. BT venom produced alterations in MAP, HR and RR. The MAP changes were seen as an immediate fall (within 2 s) followed by a rise and subsequent progressive fall. The HR was decreased drastically after venom and never returned to initial value. The respiratory changes were manifested as prolonged apnea with intermittent shallow breathing. The animals died within 30-60 min. In these animals, the lung compliance was decreased as compared to saline treated controls and there was significant increase in pulmonary water content. In aprotinin pre-treated group, there was decrease in MAP, HR and RR within 2 s which returned to pre-venom level within 15 min and remained at that level thereafter. The animals survived for the period of observation (i.e. up to 120 min). The compliance and pulmonary water content in these animals were similar to control animals. The results indicate that aprotinin protects against the BT venom-induced cardio-pulmonary toxicity.
评估了抑肽酶（激肽释放酶-激肽合成抑制剂）在预防印度锯鳞蝰蛇（BT）毒液诱导的心肺毒性中的作用。检测了BT毒液（5毫克/千克）对平均动脉压（MAP）、心率（HR）、呼吸频率（RR）、肺顺应性和肺含水量的影响。BT毒液使MAP、HR和RR发生改变。MAP的变化表现为立即下降（2秒内），随后上升，继而逐渐下降。毒液注入后HR急剧下降，且从未恢复到初始值。呼吸变化表现为长时间呼吸暂停并伴有间歇性浅呼吸。动物在30至60分钟内死亡。与生理盐水处理的对照组相比，这些动物的肺顺应性降低，肺含水量显著增加。在抑肽酶预处理组中，MAP、HR和RR在2秒内下降，15分钟内恢复到毒液注入前水平，此后保持在该水平。动物在观察期内（即长达120分钟）存活。这些动物的肺顺应性和肺含水量与对照动物相似。结果表明，抑肽酶可预防BT毒液诱导的心肺毒性。
