一种来自扇唇万代兰（Vanda spathulata (L.) Spreng.）叶尖分生组织的增殖芽培养方案。

A protocol for shoot multiplication from foliar meristem of Vanda spathulata (L.) Spreng.

作者信息

Decruse S William, Gangaprasad A, Seeni S, Menon V Sarojini

机构信息

Plant Biotechnology Division, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapuram 695 562, India.

出版信息

Indian J Exp Biol. 2003 Aug;41(8):924-7.

PMID:15248500

Abstract

Leaf explants collected from flowering plants of Vanda spathulata were cultured in Mitra medium with combinations of 6-benzyladenine (BA; 13.2-88.8 microM) and indole-3-acetic acid (IAA; 0.0 -85.6 microM). Combination of BA (66.6 microM) and IAA (28.5 microM) induced maximum shoots (17.33) from foliar meristems (leaf base). BA individually did not induce caulogenesis in leaf explants. For optimized multiplication, BA:IAA (2:1 microM) was essential at 22.2- 88.8 microM of BA. Re-cultured leaf explants produced lesser number of shoots compared to original explants and were nearly equal at combinations of 22.2-44.4 microM of BA and 5.7-28.5 microM of IAA. Rooting of shoots (> 95%) occurred in medium containing banana pulp (75 gl(-1)) and IAA (5.7 microM) within 3-9 weeks. Plantlets with 2-5 roots of 2-5 cm length established easily in community pots at 80-90% rates without hardening.

摘要

从扇唇万代兰开花植株上采集的叶片外植体，在含有6-苄基腺嘌呤（BA；13.2 - 88.8微摩尔）和吲哚-3-乙酸（IAA；0.0 - 85.6微摩尔）组合的米特拉培养基中培养。BA（66.6微摩尔）和IAA（28.5微摩尔）的组合从叶分生组织（叶基部）诱导出最多的芽（17.33个）。单独使用BA不会在叶片外植体中诱导茎的形成。为了优化增殖，在22.2 - 88.8微摩尔的BA浓度下，BA:IAA（2:1微摩尔）是必不可少的。与原始外植体相比，重新培养的叶片外植体产生的芽数量较少，在22.2 - 44.4微摩尔的BA和5.7 - 28.5微摩尔的IAA组合下，两者数量几乎相等。在含有香蕉果肉（75克/升）和IAA（5.7微摩尔）的培养基中，3 - 9周内芽的生根率达到95%以上。长度为2 - 5厘米、带有2 - 5条根的小植株在社区花盆中很容易以80 - 90%的成活率定植，无需炼苗。