Parthibhan Selvaraju, Rao Mandali Venkateswara, Senthil Kumar Thiruppathi
Department of Plant Science, Bharathidasan University, Tiruchirappalli 620 024, Tamil Nadu, India.
Department of Industry University Collaboration, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
J Genet Eng Biotechnol. 2015 Dec;13(2):227-233. doi: 10.1016/j.jgeb.2015.07.001. Epub 2015 Jul 21.
An efficient plant regeneration protocol from protocorms of was developed. The uniformly developed protocorms (o origin) having shoot initials were cultured on half macro strength MS medium (1/2 MS) supplemented with cytokinins (BA, 2iP, KIN and TDZ) at 1, 3, 5, 7, 10 mg l, natural additives (BP and CW) at 1%, 3%, 5%, 7%, 10% and auxins (IBA, NAA, 2,4-D) at 1, 3, 5, 7, 10 mg l to study their efficacy on complete plant development. A maximum of 9.4 shoots per explant were generated on 3 mg l of NAA followed by 3% of BP (7.0 shoots). Shoot elongation (1.52 cm) was achieved on 1/2 MS medium fortified with NAA 7 mg l followed by TDZ 7 mg l (1.37 cm). Shoots cultured on 1/2 MS medium supplemented with IBA 5 mg l produced an average of 8.75 roots per shoot, however the lengthiest roots (1.48 cm) were noted in NAA 7 mg l. Healthy rooted plantlets successfully acclimatized in condition. The role of complete plantlet production by natural additives could be useful for conservation and cost effective commercial production of orchids.
建立了一种从原球茎高效再生植株的方法。将具有芽原基的均匀发育的原球茎（起源）接种在添加了细胞分裂素（BA、2iP、KIN和TDZ），浓度分别为1、3、5、7、10mg/L，天然添加剂（BP和CW），浓度分别为1%、3%、5%、7%、10%，以及生长素（IBA、NAA、2,4-D），浓度分别为1、3、5、7、10mg/L的1/2强度MS培养基上，研究它们对完整植株发育的影响。在添加3mg/L NAA和3% BP的培养基上，每个外植体最多可产生9.4个芽，其次是添加3% BP的培养基（7.0个芽）。在添加7mg/L NAA和7mg/L TDZ的1/2 MS培养基上，芽伸长至1.52cm，其次是添加7mg/L TDZ的培养基（1.37cm）。在添加5mg/L IBA的1/2 MS培养基上培养的芽，每个芽平均产生8.75条根，然而，最长的根（1.48cm）出现在添加7mg/L NAA的培养基上。健康的生根小植株在该条件下成功驯化。天然添加剂在完整小植株生产中的作用可能有助于兰花的保护和经济高效的商业化生产。
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