Nagawa Fumikiyo, Hirose Satoshi, Nishizumi Hirofumi, Nishihara Tadashi, Sakano Hitoshi
Department of Biophysics and Biochemistry, Graduate School of Science, and Core Research for Evolutional Science and Technology Program of the Japan Science and Technology Agency, The University of Tokyo, Bunkyo-ku, Tokyo 113-0032, Japan.
J Biol Chem. 2004 Sep 10;279(37):38360-8. doi: 10.1074/jbc.M405485200. Epub 2004 Jul 10.
In V(D)J joining of antigen receptor genes, two recombination signal sequences (RSSs), 12- and 23-RSSs, form a complex with the protein products of recombination activating genes, RAG1 and RAG2. DNaseI footprinting demonstrates that the interaction of RAG proteins with substrate RSS DNA is not just limited to the signal region but involves the coding sequence as well. Joining mutants of RAG1 and RAG2 demonstrate impaired interactions with the coding region in both pre- and postcleavage type complexes. A possible role of this RAG coding region interaction is discussed in the context of V(D)J recombination.
在抗原受体基因的V(D)J连接过程中，两个重组信号序列（RSSs），即12-RSSs和23-RSSs，与重组激活基因RAG1和RAG2的蛋白质产物形成复合物。DNA酶I足迹分析表明，RAG蛋白与底物RSS DNA的相互作用不仅限于信号区域，还涉及编码序列。RAG1和RAG2的连接突变体在切割前和切割后类型的复合物中均表现出与编码区域的相互作用受损。本文在V(D)J重组的背景下讨论了这种RAG编码区域相互作用的可能作用。
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