Th1和Th2血清细胞因子谱可区分桥本甲状腺炎患者（Th1型）和格雷夫斯病患者（Th2型）。

Th1 and Th2 serum cytokine profiles characterize patients with Hashimoto's thyroiditis (Th1) and Graves' disease (Th2).

作者信息

Phenekos Costas, Vryonidou Andromachi, Gritzapis Angelos D, Baxevanis Constantinos N, Goula Margarita, Papamichail Michael

机构信息

Department of Endocrinology, Red Cross Hospital, Athens, Greece.

出版信息

Neuroimmunomodulation. 2004;11(4):209-13. doi: 10.1159/000078438.

DOI:10.1159/000078438

PMID:15249726

Abstract

OBJECTIVES

The aim of this study was to document the pattern of immune response, assessed by the measurement of both Th1 and Th2 serum cytokines, in patients suffering from autoimmune thyroid disease and toxic nodular goiter.

METHODS

Both Th1 and Th2 serum cytokine levels were assayed in patients suffering from Graves' disease (GD, n = 25), Hashimoto's thyroiditis (HT, n = 21), and toxic nodular goiter (TNG, n = 7) and compared with corresponding levels of 25 healthy controls. Serum concentrations of IL-2, IL-1 beta, INF-gamma, TNF-alpha, IL-12, IL-15, IL-10, IL-18, IL-4 and IL-5 were assayed in fasting serum samples.

RESULTS

It was found that patients with HT had higher IL-2 serum levels (12.16 +/- 0.66 pg/ml) compared to patients with TNG (9.25 +/- 0.84 pg/ml), GD (7.86 +/- 0.30 pg/ml) and controls (7.36 +/- 0.45 pg/ml; p = 0.0001), higher INF-gamma levels (7.60 +/- 0.33 pg/ml) compared to patients with TNG (5.77 +/- 0.55 pg/ml), GD (5.74 +/- 0.24 pg/ml) and controls (5.09 +/- 0.27 pg/ml; p = 0.0009), higher IL-12 levels (3.57 +/- 0.19 pg/ml) compared to patients with TNG (2.57 +/- 0.21 pg/ml), GD (2.48 +/- 0.13 pg/ml) and controls (2.59 +/- 0.23 pg/ml; p = 0.004), and higher IL-18 levels (27.52 +/- 1.75 pg/ml) compared to patients with TNG (18.71 +/- 2.24 pg/ml), GD (15.44 +/- 1.39 pg/ml) and controls (15.16 +/- 1.62 pg/ml; p = 0.0002). In contrast, patients with GD had higher serum levels of IL-4 (4.11 +/- 0.33 pg/ml) compared to patients with HT (3.0 +/- 0.16; p = 0.02) and higher IL-5 levels (4.22 +/- 0.30 pg/ml) compared to patients with TNG (3.21 +/- 0.58 pg/ml), HT (2.75 +/- 0.16 pg/ml) and controls (2.0 +/- 0.19 pg/ml; p = 0.0001). Patients had lower IL-1 beta serum levels (TNG 2.45 +/- 0.20, HT 2.52 +/- 0.14, GD 2.68 +/- 0.12 pg/ml) compared to controls (3.6 +/- 0.20 pg/ml; p = 0.008).

CONCLUSIONS

The above findings suggest that a Th1 pattern of immune response characteristic of cellular immunity is dominant in HT, whereas the predominance of Th2 cytokines in GD indicates a humoral pattern of immune reaction.

摘要

目的

本研究旨在通过检测自身免疫性甲状腺疾病和毒性结节性甲状腺肿患者血清中Th1和Th2细胞因子，记录其免疫反应模式。

方法

检测25例格雷夫斯病（GD）患者、21例桥本甲状腺炎（HT）患者和7例毒性结节性甲状腺肿（TNG）患者血清中Th1和Th2细胞因子水平，并与25例健康对照者的相应水平进行比较。检测空腹血清样本中白细胞介素-2（IL-2）、白细胞介素-1β（IL-1β）、干扰素-γ（INF-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素-12（IL-12）、白细胞介素-15（IL-15）、白细胞介素-10（IL-10）、白细胞介素-18（IL-18）、白细胞介素-4（IL-4）和白细胞介素-5（IL-5）的血清浓度。

结果

发现HT患者血清IL-2水平（12.16±0.66 pg/ml）高于TNG患者（9.25±0.84 pg/ml）、GD患者（7.86±0.30 pg/ml）和对照者（7.36±0.45 pg/ml；p = 0.0001）；INF-γ水平（7.60±0.33 pg/ml）高于TNG患者（5.77±0.55 pg/ml）、GD患者（5.74±0.24 pg/ml）和对照者（5.09±0.27 pg/ml；p = 0.0009）；IL-12水平（3.