佛波酯诱导蛋白激酶Cε在成纤维细胞中向细胞核易位：细胞核中与蛋白激酶Cε结合蛋白的鉴定

Phorbol ester-induced translocation of PKC epsilon to the nucleus in fibroblasts: identification of nuclear PKC epsilon-associating proteins.

作者信息

Xu Tian-Rui, Rumsby Martin G

机构信息

Department of Biology, University of York, York YO10 5DD, UK.

出版信息

FEBS Lett. 2004 Jul 16;570(1-3):20-4. doi: 10.1016/j.febslet.2004.05.080.

DOI:10.1016/j.febslet.2004.05.080

PMID:15251432

Abstract

We show that phorbol ester treatment of NIH 3T3 fibroblasts induces rapid translocation of PKC from a perinuclear site to the nucleus, extending findings in PC12 and NG108-15 cells and in myocytes. We have immunoprecipitated the PKC from nuclei isolated from phorbol ester-treated fibroblasts and identified six proteins which associate with nuclear PKC. These have been characterised as matrin 3, transferrin, Rac GTPase activating protein 1, vimentin, beta-actin and annexin II by MALDI-TOF-MS. We have confirmed that these proteins associate with PKC by gel overlay and/or dot blotting assays. The role of these PKC-associating proteins in the nucleus and their interaction with PKC are considered.

摘要

我们发现，佛波酯处理NIH 3T3成纤维细胞可诱导蛋白激酶C（PKC）从核周部位迅速转位至细胞核，这扩展了在PC12和NG108-15细胞以及肌细胞中的研究结果。我们从经佛波酯处理的成纤维细胞分离出的细胞核中免疫沉淀了PKC，并鉴定出六种与核PKC相关的蛋白质。通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS），这些蛋白质已被鉴定为核基质蛋白3、转铁蛋白、Rac GTP酶激活蛋白1、波形蛋白、β-肌动蛋白和膜联蛋白II。我们已通过凝胶覆盖和/或斑点印迹分析证实这些蛋白质与PKC相关。我们考虑了这些与PKC相关的蛋白质在细胞核中的作用及其与PKC的相互作用。