Nowak-Markwitz Ewa, Jankowska Anna, Szczerba Anna, Andrusiewicz Miroslaw, Warchoł Jerzy B
Division of Obstetric and Gynecology, University of Medical Sciences, Poznań, Poland.
Folia Histochem Cytobiol. 2004;42(2):123-6.
Recent studies demonstrated that besides placenta and malignant trophoblastic tumors, hCG and especially its beta-subunit is secreted by a varieties of tumors of different origin. The aim of the present investigation was to determine the expression pattern of human chorionic gonadotropin gene in ovarian cancer tissue. The study included 8 patients with epithelial ovarian carcinoma. The expression and distribution of hCGbeta mRNA was assessed by in situ RT-PCR method. The semi-quantitative assessment was performed using computer image analysis. Transformation of the images into the pseudocolour scale showed a clear difference in fluorescence intensity among individual cancer cells. The intensity of ISRT-PCR products corresponding with expression level of hCGbeta demonstrated that its production by individual cancer cells is different. In all studied specimens of the ovarian carcinoma tissue, cancer cells characterized by the presence of active hCGbeta gene were found, whereas noncancerous tissue demonstrated lack of the gene expression. Thus, the study clearly shows that the expression of hCGbeta is the feature of ovarian cancer tissue.
最近的研究表明，除胎盘和恶性滋养层肿瘤外，多种不同起源的肿瘤均可分泌人绒毛膜促性腺激素（hCG），尤其是其β亚基。本研究的目的是确定人绒毛膜促性腺激素基因在卵巢癌组织中的表达模式。该研究纳入了8例上皮性卵巢癌患者。采用原位逆转录聚合酶链反应（RT-PCR）法评估hCGβ mRNA的表达和分布。使用计算机图像分析进行半定量评估。将图像转换为伪彩色标度显示，各个癌细胞之间的荧光强度存在明显差异。与hCGβ表达水平相对应的原位RT-PCR产物强度表明，单个癌细胞的hCGβ产生量不同。在所有研究的卵巢癌组织标本中，均发现了具有活跃hCGβ基因的癌细胞，而非癌组织则未显示该基因表达。因此，该研究清楚地表明，hCGβ的表达是卵巢癌组织的一个特征。
