[血管内皮生长因子基因转染与大鼠随意皮瓣存活]

[Vascular endothelial growth factor gene transfection and survival of the random skin flap in rats].

作者信息

Xiong Bing, Yi Chuan-xun, Chen Hua-de, Guo Jian-li, Zhang Yi-ming, Feng Xiao-ling, Zhou Wen-dong

机构信息

Department of Burns, People's Hospital of Guangdong Province, Guangzhou 510080, China.

出版信息

Di Yi Jun Yi Da Xue Xue Bao. 2004 Jul;24(7):794-7.

PMID:15257906

Abstract

OBJECTIVE

To investigate the effects of vascular endothelial growth factor (VEGF) gene transfection on survival of the random skin flap in rats.

METHOD

Thirty SD rats were randomized equally into 3 groups: pcDNA3-VEGF165, pcDNA3 and control groups, with the former two groups transfected via liposome with pcDNA3-VEGF165 and pcDNA3 respectively 48 h before and during the operation. Ischemic random skin flaps ( 1 cmx7 cm) were constructed from the rats. Seven days later, the amount of viable tissue within the flap was measured by planimetry. After the animals were killed, and specimens from the random skin flaps were harvested for immunohistologic evidence of VEGF protein expression and for HE staining to examine the microvascular growth.

RESULTS

The results of tissue survival planimetry of the skin flap of pcDNA3-VEGF165, pcDNA3 and control groups were 48.46% +/-3.35%, 30.20%+/-2.16%, and 31.35% +/-1.99%, which were highest in the VEGF- transfected group (P<0.05), in which immunohistochemical staining revealed increased deposition of VEGF in comparison with the other control groups P<0.05 . The VEGF group had also higher average vessel number as compared with the vector and control group (107.72+/-9.42 vs 91.35+/-7.28 and 89.85+/-7.66, P<0.05), and smaller average vessel lumen diameter (25.76+/-3.23 microm vs 32.12+/-1.58 microm and 33.49+/-2.29 microm, P<0.05).

CONCLUSION

pcDNA3-VEGF165 transfection may enhance the survival of the ischemic skin flaps and achieve VEGF expression in the flaps in rats.

摘要

目的

探讨血管内皮生长因子（VEGF）基因转染对大鼠随意皮瓣存活的影响。

方法

30只SD大鼠随机均分为3组：pcDNA3-VEGF165组、pcDNA3组和对照组，前两组分别于手术前48小时及手术中经脂质体转染pcDNA3-VEGF165和pcDNA3。从大鼠身上构建缺血随意皮瓣（1 cm×7 cm）。7天后，通过平面测量法测量皮瓣内存活组织的量。处死动物后，采集随意皮瓣标本，进行VEGF蛋白表达的免疫组织学证据检测及HE染色以检查微血管生长情况。

结果

pcDNA3-VEGF165组、pcDNA3组和对照组皮瓣组织存活平面测量结果分别为48.46%±3.35%、30.20%±2.16%和31.35%±1.99%，VEGF转染组最高（P<0.05），免疫组织化学染色显示该组VEGF沉积较其他对照组增加（P<0.05）。VEGF组平均血管数量也高于载体组和对照组（107.72±9.42对91.35±7.28和89.85±7.66，P<0.05），平均血管腔直径较小（25.76±3.23微米对32.12±1.58微米和33.49±2.29微米，P<0.05）。