Boursnell J C, Noble E A, Andrews M G
J Reprod Fertil. 1975 Dec;45(3):415-20. doi: 10.1530/jrf.0.0450415.
About 30% of boar seminal plasma nitrogen is maximally precipitated at room temperature by 6 to 10 mM zinc in citrate solution at pH 8. A rise in the total nitrogen precipitated by 1 to 6 mM zinc is accompanied by a fall in the haemagglutinin titre of the supernatant fluid. At 6 mM zinc addition, 95% of the haemagglutinin is precipitated, but much of this is recoverable by re-solution of the zinc precipitate. Protein profile studies by gel-filtration chromatography of the zinc precipitate solution reveals a mixture of proteins, some of which are not by themselves zinc-precipitable.
约30%的公猪精浆氮在室温下，被pH值为8的柠檬酸盐溶液中6至10 mM的锌最大程度沉淀。1至6 mM锌沉淀的总氮量增加时，上清液的血凝素效价会下降。添加6 mM锌时，95%的血凝素会沉淀，但其中大部分可通过锌沉淀物的再溶解回收。对锌沉淀物溶液进行凝胶过滤色谱法的蛋白质谱研究显示为蛋白质混合物，其中一些蛋白质本身不能被锌沉淀。
