微阵列形式的多重夹心测定法。

Multiplexed sandwich assays in microarray format.

作者信息

Nielsen Ulrik B, Geierstanger Bernhard H

机构信息

Merrimack Pharmaceuticals, 101 Binney Street, Cambridge, MA 02142, USA.

出版信息

J Immunol Methods. 2004 Jul;290(1-2):107-20. doi: 10.1016/j.jim.2004.04.012.

DOI:10.1016/j.jim.2004.04.012

PMID:15261575

Abstract

Antibody arrays have evolved into powerful tools for quantifying proteins and qualifying their state of activation in complex biological samples. This level of analysis holds tremendous promise as part of a diagnostic or prognostic platform. In particular, multiplex sandwich ELISA assays performed with microarrays in the wells of multi-well plates enable high-throughput analysis of multiple samples with multivariate readouts. Here, we compare and review recent advances in antibody microarray technology and describe its promises towards the systematic analysis of complex biological samples, ranging from profiling patient sera to studying intracellular signaling.

摘要

抗体阵列已发展成为用于定量蛋白质并确定其在复杂生物样品中激活状态的强大工具。作为诊断或预后平台的一部分，这种分析水平具有巨大的前景。特别是，在多孔板孔中使用微阵列进行的多重夹心ELISA检测能够对多个样品进行高通量分析，并提供多变量读数。在这里，我们比较并回顾了抗体微阵列技术的最新进展，并描述了其在系统分析复杂生物样品方面的前景，从分析患者血清到研究细胞内信号传导。