Okeoma C M, Williamson N B, Pomroy W E, Stowell K M, Gillespie L
Institute of Veterinary Animal and Biological Sciences, Massey University, Palmerston North, New Zealand.
Vet Parasitol. 2004 Aug 6;122(4):307-15. doi: 10.1016/j.vetpar.2004.06.001.
Twelve 2-year old heifers in their fifth month of gestation when pregnancy tested were used in this study. Six heifers aborted at approximately 4 months of gestation and had blood samples drawn less than 6 weeks after the abortions were identified. Blood samples were also drawn from three sero-positive pregnant and three sero-negative pregnant heifers. DNA was isolated from the samples and a 350 bp fragment of the Nc-5 gene was PCR amplified using primer pair Np21+ and Np6+. Also, the Internal Transcribed Spacer 1 (ITS1) was PCR amplified using Tim 3 and Tim 11 primer pair. The Nc-5 gene fragment was cloned, sequenced and the sequence BLAST-tested. Similarly, the ITS1 product was sequenced and BLAST-tested. The BLAST test results revealed that Neospora caninum DNA was present in these blood samples indicating that polymerase chain reaction can be used in the detection of N. caninum DNA in the blood of sero-positive cows.
本研究使用了12头妊娠5个月、经妊娠检测为2岁的小母牛。6头小母牛在妊娠约4个月时流产，并在确认流产后不到6周采集血样。还从3头血清阳性妊娠小母牛和3头血清阴性妊娠小母牛采集血样。从样本中分离DNA，使用引物对Np21 +和Np6 +对Nc - 5基因的350 bp片段进行PCR扩增。此外，使用Tim 3和Tim 11引物对扩增内部转录间隔区1（ITS1）。对Nc - 5基因片段进行克隆、测序并进行序列BLAST检测。同样，对ITS1产物进行测序并进行BLAST检测。BLAST检测结果显示这些血样中存在犬新孢子虫DNA，表明聚合酶链反应可用于检测血清阳性奶牛血液中的犬新孢子虫DNA。
Zotero 插件