携带大肠杆菌乳糖和半乳糖操纵子的广宿主范围质粒。

Broad host range plasmids carrying the Escherichia coli lactose and galactose operons.

作者信息

Lodge J, Fear J, Busby S, Gunasekaran P, Kamini N R

机构信息

School of Biochemistry, University of Birmingham, UK.

出版信息

FEMS Microbiol Lett. 1992 Aug 15;74(2-3):271-6. doi: 10.1016/0378-1097(92)90441-p.

DOI:10.1016/0378-1097(92)90441-p

PMID:1526459

Abstract

We have developed a number of broad-host-range plasmids that allow the expression of the Escherichia coli lac operon from any cloned promoter, and the creation of 'in phase' fusions between lacZ and other cloned genes. In a second series of constructions, the E. coli gal operon has been cloned into the broad-host-range vector and a plasmid carrying both the E. coli gal and lac genes is described. These plasmids have been transferred into Pseudomonas aeruginosa and Zymomonas mobilis and their effects on the utilisation of lactose and galactose have been investigated.

摘要

我们已经构建了多种广宿主范围质粒，这些质粒能够使大肠杆菌乳糖操纵子从任何克隆的启动子表达，并能在lacZ与其他克隆基因之间构建“同框”融合。在第二系列构建中，大肠杆菌半乳糖操纵子已被克隆到广宿主范围载体中，并描述了一种携带大肠杆菌半乳糖和乳糖基因的质粒。这些质粒已被转入铜绿假单胞菌和运动发酵单胞菌，并研究了它们对乳糖和半乳糖利用的影响。