大肠杆菌lacZ和lacY基因的克隆及其在氧化葡萄糖酸杆菌和液化醋杆菌中的表达。
Cloning of Escherichia coli lacZ and lacY genes and their expression in Gluconobacter oxydans and Acetobacter liquefaciens.
作者信息
Mostafa Hesham E, Heller Knut J, Geis Arnold
机构信息
Institute for Microbiology, Federal Dairy Research Centre, 24103 Kiel, Germany.
出版信息
Appl Environ Microbiol. 2002 May;68(5):2619-23. doi: 10.1128/AEM.68.5.2619-2623.2002.
Abstract
An efficient transformation protocol for Gluconobacter oxydans and Acetobacter liquefaciens strains was developed by preparation of electrocompetent cells grown on yeast extract-ethanol medium. Plasmid pBBR122 was used as broad-host-range vector to clone the Escherichia coli lacZY genes in G. oxydans and A. liquefaciens. Although both lac genes were functionally expressed in both acetic acid bacteria, only a few transformants were able to grow on lactose. However, this ability strictly depended on the presence of a plasmid expressing both lac genes. Mutations in the plasmids and/or in the chromosome were excluded as the cause of growth ability on lactose.
摘要
通过制备在酵母提取物 - 乙醇培养基上生长的电转化感受态细胞，开发了一种用于氧化葡萄糖杆菌和液化醋杆菌菌株的高效转化方案。质粒pBBR122用作广宿主范围载体，以在氧化葡萄糖杆菌和液化醋杆菌中克隆大肠杆菌lacZY基因。尽管两个lac基因在两种醋酸菌中都有功能表达，但只有少数转化体能够在乳糖上生长。然而，这种能力严格依赖于表达两个lac基因的质粒的存在。排除了质粒和/或染色体中的突变作为在乳糖上生长能力的原因。
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Cloning of Escherichia coli lacZ and lacY genes and their expression in Gluconobacter oxydans and Acetobacter liquefaciens.大肠杆菌lacZ和lacY基因的克隆及其在氧化葡萄糖酸杆菌和液化醋杆菌中的表达。
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