Azevedo Ricardo A, Lea Peter J, Damerval Catherine, Landry Jacques, Bellato Cláudia M, Meinhardt Lyndel W, Le Guilloux Martine, Delhaye Sonia, Varisi Vanderlei A, Gaziola Salete A, Gratão Priscila L, Toro Alejandro A
Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, and Centro de Energia Nuclear na Agricultura, Universidade de São Paulo, Piracicaba CEP 13418-900, SP, Brazil.
J Agric Food Chem. 2004 Jul 28;52(15):4865-71. doi: 10.1021/jf035422h.
Two high lysine maize endosperm mutations, opaque-5 (o5) and opaque-7 (o7), were biochemically characterized for endosperm protein synthesis and lysine metabolism in immature seeds. Albumins, globulins, and glutelins, which have a high content of lysine, were shown to be increased in the mutants, whereas zeins, which contain trace concentrations of lysine, were reduced in relation to the wild-type lines B77xB79+ and B37+. These alterations in the storage protein fraction distribution possibly explain the increased concentration of lysine in the two mutants. Using two-dimensional polyacrylamide gel electrophoresis of proteins of mature grains, variable amounts of zein polypeptides were detected and considerable differences were noted between the four lines studied. The analysis of the enzymes involved in lysine metabolism indicated that both mutants have reduced lysine catabolism when compared to their respective wild types, thus allowing more lysine to be available for storage protein synthesis.
对两个高赖氨酸玉米胚乳突变体，不透明-5（o5）和不透明-7（o7），就未成熟种子中胚乳蛋白质合成和赖氨酸代谢进行了生化特性分析。在突变体中，富含赖氨酸的清蛋白、球蛋白和谷蛋白含量增加，而含微量赖氨酸的醇溶蛋白相对于野生型品系B77xB79+和B37+有所减少。贮藏蛋白组分分布的这些变化可能解释了这两个突变体中赖氨酸浓度的增加。通过对成熟籽粒蛋白质进行二维聚丙烯酰胺凝胶电泳，检测到了不同数量的醇溶蛋白多肽，并且在所研究的四个品系之间发现了显著差异。对参与赖氨酸代谢的酶的分析表明，与各自的野生型相比，这两个突变体的赖氨酸分解代谢均有所降低，从而使更多的赖氨酸可用于贮藏蛋白的合成。
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