Wu Peizhen, Yuan Yanli, Ma Zhoujie, Zhang Kaiwu, Deng Lei, Ren Hong, Yang Wenpeng, Wang Wei
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, Guizhou Province, China.
Guizhou Institute of Upland Food Crops, Guizhou Academy of Agricultural Sciences, Guiyang 550006, Guizhou Province, China.
Breed Sci. 2024 Sep;74(4):311-323. doi: 10.1270/jsbbs.23051. Epub 2024 Aug 14.
To explore the molecular mechanism behind maize grain quality and use of different gene stacking to improve the nutritional quality of grain, marker-assisted selection (MAS) was used to select three recessive mutant lines containing , along with the double-recessive mutant lines containing , , and . The resulting seeds were taken for transcriptome sequencing analysis 18 days after pollination (DAP). Results: Compared with the recurrent parent genes, in the lysine synthesis pathway, the gene pyramiding lines (, , and ) revealed that the gene encoding aspartate kinase (AK) was up-regulated and promoted lysine synthesis. In the lysine degradation pathway, 'QCL8010_1' () revealed that the gene encoding saccharopine dehydrogenase (LKR/SDH) was down-regulated. In addition, the gene pyramiding lines (, , and ) indicated that the gene encoding 2-oxoglutarate dehydrogenase E1 component (OGDH) was down-regulated, inhibiting the degradation of lysine. In the tryptophan synthesis pathway, the genes encoding anthranilate synthase (AS), anthranilate synthase (APT), and tryptophan synthase (TS) were up-regulated (in , , , and ), and promote tryptophan synthesis. In the tryptophan degradation pathway, it was revealed that the genes encoding indole-3-producing oxidase (IAAO) and indole-3-pyruvate monooxygenase (YUCCA) were down-regulated. These results provide a reference for revealing the mechanism of the , , and with different gene pyramiding to improve grain quality in maize.
为探究玉米籽粒品质背后的分子机制以及利用不同基因叠加来改善籽粒营养品质，采用标记辅助选择（MAS）方法筛选出三个含有[具体基因]的隐性突变系，以及含有[其他具体基因]的双隐性突变系。对授粉后18天（DAP）的所得种子进行转录组测序分析。结果：与轮回亲本基因相比，在赖氨酸合成途径中，基因叠加系（[具体叠加系名称]）显示编码天冬氨酸激酶（AK）的基因上调，促进了赖氨酸合成。在赖氨酸降解途径中，‘QCL8010_1’（[具体基因情况]）显示编码酵母氨酸脱氢酶（LKR/SDH）的基因下调。此外，基因叠加系（[具体叠加系名称]）表明编码2-氧代戊二酸脱氢酶E1组分（OGDH）的基因下调，抑制了赖氨酸的降解。在色氨酸合成途径中，编码邻氨基苯甲酸合酶（AS）、邻氨基苯甲酸磷酸核糖转移酶（APT）和色氨酸合酶（TS）的基因上调（在[具体情况]中），促进了色氨酸合成。在色氨酸降解途径中，显示编码吲哚-3-生成氧化酶（IAAO）和吲哚-3-丙酮酸单加氧酶（YUCCA）的基因下调。这些结果为揭示[具体基因情况]不同基因叠加改善玉米籽粒品质的机制提供了参考。
