在丙酮中可控区域选择性酶促合成可聚合的5'-O-乙烯基和3'-O-乙烯基核苷类似物。
Controllable regioselective enzymatic synthesis of polymerizable 5'-O-vinyl- and 3'-O-vinyl-nucleoside analogues in acetone.
作者信息
Sun Xiao-Feng, Wang Na, Wu Qi, Lin Xian-Fu
机构信息
Department of Chemistry, Zhejiang University, Hangzhou 310027, P.R. China.
出版信息
Biotechnol Lett. 2004 Jun;26(12):1019-22. doi: 10.1023/b:bile.0000030050.20353.a6.
Abstract
An efficient synthesis of polymerizable 3'- and 5'-O-acyl-nucleoside derivatives has been developed from inosine and 2'-deoxyuridine by enzyme-catalyzed regioselective acylation with divinyl dicarboxylates. In acetone, Lipozyme (immobilized lipase from Mucor miehei) gave 5'-O-acyl-nucleoside products, and PPL (lipase from porcine pancreas) provided 3'-O-acyl-nucleoside products.
摘要
通过用二乙烯基二羧酸酯进行酶催化区域选择性酰化反应，从肌苷和2'-脱氧尿苷出发，已开发出一种高效合成可聚合的3'-和5'-O-酰基核苷衍生物的方法。在丙酮中，Lipozyme（米黑根毛霉固定化脂肪酶）生成5'-O-酰基核苷产物，而PPL（猪胰脂肪酶）则提供3'-O-酰基核苷产物。
Zotero 插件