Duran R, Chion C K, Bigey F, Arnaud A, Galzy P
Chaire de Microbiologie Industrielle et de Génétique des Microorganismes, Ecole Nationale Supérieure Agronomique de Montpellier, France.
J Basic Microbiol. 1992;32(1):13-9. doi: 10.1002/jobm.3620320104.
Nitrile hydratase from Brevibacterium sp. R312 was purified to homogeneity. The isoelectric point was 5.75. The two kinds of subunits were separated by reverse phase HPLC and their N-terminal amino acid sequences were found to be identical to those of Rhodococcus sp. N-774 nitrile hydratase.
从短杆菌属R312中纯化得到了腈水合酶，使其达到了均一性。其等电点为5.75。通过反相高效液相色谱法分离出了两种亚基，发现它们的N端氨基酸序列与红球菌属N-774腈水合酶的N端氨基酸序列相同。
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