用于红球菌属R312（以前称为短杆菌属R312）的新型穿梭载体，一种产腈水合酶的菌株。

New shuttle vectors for Rhodococcus sp. R312 (formerly Brevibacterium sp. R312), a nitrile hydratase producing strain.

作者信息

Duran R

机构信息

Laboratoire d'Ecologie Moléculaire, I.B.E.A.S., Université de Pau et des Pays de l'Adour, France.

出版信息

J Basic Microbiol. 1998;38(2):101-6.

PMID:9637010

Abstract

Two shuttle vectors named pRC52 (10.7 Kb) and pRK52 (12.2 Kb) carrying chloramphenicol (Cm) and chloramphenicol plus kanamycin (Km) resistance genes, respectively, were constructed by fusion of a cryptic plasmid pBL13869 (replicon pBL1, 5.8 Kb) from Brevibacterium lactofermentum ATCC13869 with pBR328 E. coli plasmid. Transformation of Rhodococcus sp. R312 (formerly Brevibacterium sp. R312) protoplasts was realised with an efficiency of 28 transformants per micrograms of DNA.

摘要

构建了两个穿梭载体，分别命名为pRC52（10.7 Kb）和pRK52（12.2 Kb），它们分别携带氯霉素（Cm）抗性基因和氯霉素加卡那霉素（Km）抗性基因。这两个载体是通过将来自乳酸发酵短杆菌ATCC13869的隐蔽质粒pBL13869（复制子pBL1，5.8 Kb）与大肠杆菌质粒pBR328融合构建而成的。用每微克DNA可获得28个转化体的效率实现了红球菌属R312（以前称为短杆菌属R312）原生质体的转化。