利用嗜酸性粒细胞趋化因子-单核细胞趋化蛋白-1嵌合体鉴定趋化因子识别的特异性决定因素。

Specificity determinants for chemokine recognition identified using eotaxin-MCP-1 chimeras.

作者信息

Mayer Michael R, Parody Todd R, Datta-Mannan Amita, Stone Martin J

机构信息

Department of Chemistry, Indiana University, Bloomington, IN 47405-0001, USA.

出版信息

FEBS Lett. 2004 Jul 30;571(1-3):166-70. doi: 10.1016/j.febslet.2004.06.079.

DOI:10.1016/j.febslet.2004.06.079

PMID:15280036

Abstract

To identify the elements of two chemokines [monocyte chemoattractant protein-1 (MCP-1) and eotaxin] that control their differential recognition by their respective receptors (CCR2 and CCR3), we have studied the receptor interactions of MCP-1-eotaxin chimeras. Each receptor was found to exhibit a distinct binding preference for proteins containing the amino-terminal region of the cognate chemokine for that receptor. However, other elements dictating chemokine preference were different for the two receptors. In some cases, the influence of replacing a particular region was dependent on the identities of neighboring regions, indicating a complex network of cooperative and/or compensating interactions.

摘要

为了确定两种趋化因子[单核细胞趋化蛋白-1（MCP-1）和嗜酸性粒细胞趋化因子]中控制它们被各自受体（CCR2和CCR3）差异性识别的元件，我们研究了MCP-1-嗜酸性粒细胞趋化因子嵌合体与受体的相互作用。发现每个受体对含有该受体同源趋化因子氨基末端区域的蛋白质表现出独特的结合偏好。然而，决定趋化因子偏好的其他元件在两种受体中是不同的。在某些情况下，替换特定区域的影响取决于相邻区域的特性，这表明存在一个复杂的协同和/或补偿相互作用网络。