利用源自马传染性贫血病毒（EIAV）的载体高效生成转基因猪。

Efficient generation of transgenic pigs using equine infectious anaemia virus (EIAV) derived vector.

作者信息

Whitelaw C Bruce A, Radcliffe Pippa A, Ritchie William A, Carlisle Ailsa, Ellard Fiona M, Pena Romi N, Rowe Jo, Clark A John, King Tim J, Mitrophanous Kyriacos A

机构信息

Department of Gene Function and Development, Roslin Institute, Roslin, Midlothian EH25 9PS, UK.

出版信息

FEBS Lett. 2004 Jul 30;571(1-3):233-6. doi: 10.1016/j.febslet.2004.06.076.

DOI:10.1016/j.febslet.2004.06.076

PMID:15280048

Abstract

Traditional methods of transgene delivery in livestock are inefficient. Recently, human immunodeficiency virus (HIV-1) based lentiviral vectors have been shown to offer an efficient transgene delivery system. We now extend this method by demonstrating efficient generation of transgenic pigs using an equine infectious anaemia virus derived vector. We used this vector to deliver a green fluorescent protein expressing transgene; 31% of injected/transferred eggs resulted in a transgenic founder animal and 95% of founder animals displayed green fluorescence. This compares favourably with results using HIV-1 based vectors, and is substantially more efficient than the standard pronuclear microinjection method, indicating that lentiviral transgene delivery may be a general tool with which to efficiently generate transgenic mammals.

摘要

家畜中传统的转基因递送方法效率低下。最近，基于人类免疫缺陷病毒（HIV-1）的慢病毒载体已被证明可提供一种高效的转基因递送系统。我们现在通过使用源自马传染性贫血病毒的载体展示高效生成转基因猪来扩展这种方法。我们使用该载体递送表达绿色荧光蛋白的转基因；31%的注射/转移卵子产生了转基因奠基动物，95%的奠基动物显示出绿色荧光。这与使用基于HIV-1的载体的结果相比具有优势，并且比标准的原核显微注射方法效率高得多，表明慢病毒转基因递送可能是一种有效生成转基因哺乳动物的通用工具。