Chan A W, Chong K Y, Martinovich C, Simerly C, Schatten G
Oregon Regional Primate Research Center, Center for Women's Health, and Departments of Cell-Developmental Biology and Obstetrics-Gynecology, Oregon Health Sciences University, 505 NW 185th Avenue, Beaverton, OR 97006, USA.
Science. 2001 Jan 12;291(5502):309-12. doi: 10.1126/science.291.5502.309.
Transgenic rhesus monkeys carrying the green fluorescent protein (GFP) gene were produced by injecting pseudotyped replication-defective retroviral vector into the perivitelline space of 224 mature rhesus oocytes, later fertilized by intracytoplasmic sperm injection. Of the three males born from 20 embryo transfers, one was transgenic when accessible tissues were assayed for transgene DNA and messenger RNA. All tissues that were studied from a fraternal set of twins, miscarried at 73 days, carried the transgene, as confirmed by Southern analyses, and the GFP transgene reporter was detected by both direct and indirect fluorescence imaging.
通过将假型复制缺陷型逆转录病毒载体注射到224个成熟恒河猴卵母细胞的卵周隙中，随后通过胞浆内单精子注射使其受精，从而培育出携带绿色荧光蛋白（GFP）基因的转基因恒河猴。在20次胚胎移植所出生的3只雄性恒河猴中，当对可获取的组织进行转基因DNA和信使RNA检测时，有1只是转基因猴。对一组73天流产的异卵双胞胎所研究的所有组织进行Southern分析证实均携带转基因，并且通过直接和间接荧光成像检测到了GFP转基因报告基因。
