插入P转座子中的Su(Hw)绝缘子的Mod(mdg4)组件可抑制其在黑腹果蝇中的移动性。
The Mod(mdg4) component of the Su(Hw) insulator inserted in the P transposon can repress its mobility in Drosophila melanogaster.
作者信息
Karakozova Marina, Savitskaya Ekaterina, Melnikova Larisa, Parshikov Aleksandr, Georgiev Pavel
机构信息
Department of the Control of Genetic Processes, Institute of Gene Biology, Russian Academy of Sciences, Moscow 119334, Russia.
出版信息
Genetics. 2004 Jul;167(3):1275-80. doi: 10.1534/genetics.104.027037.
Abstract
Transposable element P of Drosophila melanogaster is one of the best-characterized eukaryotic transposons. Successful transposition requires the interaction between transposase complexes at both termini of the P element. Here we found that insertion of one or two copies of the Su(Hw) insulator in the P transposon reduces the frequency of its transposition. Inactivation of a Mod(mdg4) component of the Su(Hw) insulator suppresses the insulator effect. Thus, the Su(Hw) insulator can modulate interactions between transposase complexes bound to the ends of the P transposon in germ cells.
摘要
果蝇的转座因子P是特征最明确的真核转座子之一。成功的转座需要P因子两端的转座酶复合物之间相互作用。我们发现，在P转座子中插入一或两个拷贝的Su(Hw)绝缘子会降低其转座频率。Su(Hw)绝缘子的Mod(mdg4)组分失活会抑制绝缘子效应。因此，Su(Hw)绝缘子可以调节生殖细胞中与P转座子末端结合的转座酶复合物之间的相互作用。
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