Scheldeman Patsy, Rodríguez-Díaz Marina, Goris Johan, Pil Annelies, De Clerck Elke, Herman Lieve, De Vos Paul, Logan Niall A, Heyndrickx Marc
Ministry of the Flemish Community, Centre for Agricultural Research, Department of Animal Product Quality, Brusselsesteenweg 370, 9090 Melle, Belgium.
Glasgow Caledonian University, School of Biological and Biomedical Sciences, Cowcaddens Road, Glasgow G4 0BA, UK.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1355-1364. doi: 10.1099/ijs.0.63095-0.
Forty-eight bacterial strains were isolated at dairy farms from raw milk, the milking apparatus, green fodder or feed concentrate after a heat treatment of 30 min at 100 degrees C. In this way, spore-forming bacteria with a very high intrinsic heat resistance were selected for. The aerobic spore-forming isolates were subjected to a polyphasic taxonomical study, including repetitive element sequence-based PCR typing, whole-cell protein profiling, 16S rDNA sequence analysis, DNA-DNA hybridizations, DNA base composition, fatty acid analysis, and morphological and biochemical characteristics. A comparison of the REP- and (GTG)5-PCR and whole-cell protein SDS-PAGE profiles resulted in three clusters of similar strains. Analysis of the 16S rDNA sequences and DNA-DNA relatedness data showed that these clusters represented three novel species. The highest 16S rDNA similarity to a recognized species found for the three groups was around 94% with Bacillus lentus and Bacillus sporothermodurans. Further phenotypic characterization supported the proposal of three novel species in the genus Bacillus, Bacillus farraginis, Bacillus fortis and Bacillus fordii. The respective type strains are R-6540T (=LMG 22081T=DSM 16013T), R-6514T (=LMG 22079T=DSM 16012T) and R-7190T (=LMG 22080T=DSM 16014T); their G+C DNA base contents are 43.7, 44.3 and 41.9 mol%, respectively. Although in variable amounts, a predominance of the branched fatty acids iso-C(15 : 0) and anteiso-C(15 : 0) was observed in all three novel species.
在奶牛场，从生牛奶、挤奶设备、青饲料或经过100℃ 30分钟热处理的精饲料中分离出48株细菌菌株。通过这种方式，筛选出了具有非常高固有耐热性的芽孢杆菌。对需氧芽孢杆菌分离株进行了多相分类研究，包括基于重复元件序列的PCR分型、全细胞蛋白质谱分析、16S rDNA序列分析、DNA-DNA杂交、DNA碱基组成、脂肪酸分析以及形态和生化特征分析。REP-PCR和(GTG)5-PCR以及全细胞蛋白质SDS-PAGE图谱的比较产生了三个相似菌株簇。16S rDNA序列分析和DNA-DNA相关性数据表明，这些簇代表三个新物种。这三组菌株与已确认物种的最高16S rDNA相似性约为94%，与迟缓芽孢杆菌和嗜热栖热芽孢杆菌相似。进一步的表型特征支持了芽孢杆菌属中三个新物种的提议——Farraginis芽孢杆菌、Fortis芽孢杆菌和Fordii芽孢杆菌。各自的模式菌株分别为R-6540T（=LMG 22,081T=DSM 16,013T）、R-6514T（=LMG 22,079T=DSM 16,012T）和R-7190T（=LMG 22,080T=DSM 16,014T）；它们的G+C DNA碱基含量分别为43.7、44.3和41.9 mol%。尽管含量不同，但在所有这三个新物种中均观察到支链脂肪酸iso-C(15 : 0)和anteiso-C(15 : 0)占主导地位。
