Logan Niall A, De Clerck Elke, Lebbe Liesbeth, Verhelst An, Goris Johan, Forsyth Gillian, Rodríguez-Díaz Marina, Heyndrickx Marc, De Vos Paul
School of Biological and Biomedical Sciences, Glasgow Caledonian University, Cowcaddens Road, Glasgow G4 0BA, UK.
Vakgroep BFM WE10V, Laboratorium voor Microbiologie, Universiteit Gent, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1071-1076. doi: 10.1099/ijs.0.02967-0.
Seven strains of aerobic, endospore-forming bacteria were found in soil taken from an active fumarole on Lucifer Hill, Candlemas Island, South Sandwich archipelago, Antarctica, and four strains were from soil of an inactive fumarole at the foot of the hill. Amplified rDNA restriction analysis, 16S rDNA sequence comparisons, SDS-PAGE and routine phenotypic tests support the proposal of two novel species of Paenibacillus, Paenibacillus cineris sp. nov. and Paenibacillus cookii sp. nov., the type strains of which are LMG 18439T (=CIP 108109T) and LMG 18419T (=CIP 108110T), respectively. A further strain, isolated from a gelatin-production process, showed more than 99% 16S rDNA sequence similarity to the proposed P. cookii type strain and, although the gelatin isolate was atypical when compared with the fumarole isolates by repeated element primed-PCR, SDS-PAGE and phenotypic analyses, it was shown by DNA-DNA reassociation studies to belong to the same species. Strains of P. cookii produce spreading growth with motile microcolonies. Both species produce swollen sporangia that are typical for the genus, they both show 97.6% 16S rDNA sequence similarity to Paenibacillus azoreducens, they have 51.5-51.6 mol% G+C in their DNA and their major fatty acid is anteiso-C(15 : 0); however, fatty acids C(16 : 0) and anteiso-C(17 : 0) represent, respectively, 18 and 10 % of the total in P. cineris, but 11 and 20% in P. cookii.
在取自南极洲南桑威奇群岛坎德马斯岛路西法山一个活跃喷气孔的土壤中发现了7株需氧、产芽孢细菌，另外4株来自山脚下一个不活跃喷气孔的土壤。扩增核糖体DNA限制酶切分析、16S rDNA序列比较、SDS-PAGE和常规表型测试支持了两个新种芽孢杆菌属细菌的提议，即灰质芽孢杆菌（Paenibacillus cineris sp. nov.）和库克芽孢杆菌（Paenibacillus cookii sp. nov.），它们的模式菌株分别为LMG 18439T（=CIP 108109T）和LMG 18419T（=CIP 108110T）。从明胶生产过程中分离出的另一株菌株，其16S rDNA序列与提议的库克芽孢杆菌模式菌株的相似性超过99%，尽管通过重复元件引发PCR、SDS-PAGE和表型分析将该明胶分离株与喷气孔分离株比较时它是非典型的，但DNA-DNA杂交研究表明它属于同一物种。库克芽孢杆菌菌株产生带有运动性小菌落的扩散生长。这两个物种都产生该属典型的膨大孢子囊，它们与偶氮还原芽孢杆菌的16S rDNA序列相似性均为97.6%，其DNA的G+C含量为51.5 - 51.6 mol%，主要脂肪酸为anteiso-C(15 : 0)；然而，脂肪酸C(16 : 0)和anteiso-C(17 : 0)在灰质芽孢杆菌中分别占总量的18%和10%，而在库克芽孢杆菌中分别占11%和20%。
