针对聚集蛋白聚糖中降解性新表位产生抗体。

Production of antibodies against degradative neoepitopes in aggrecan.

作者信息

Mort John S, Roughley Peter J

机构信息

Joint Diseases Laboratory, Shriners Hospital for Children, and Departments of Surgery and Medicine, McGill University, Montreal, Quebec, Canada.

出版信息

Methods Mol Med. 2004;100:237-50. doi: 10.1385/1-59259-810-2:237.

DOI:10.1385/1-59259-810-2:237

PMID:15280599

Abstract

The use of synthetic peptides to generate rabbit polyclonal anticatabolic neoepitope antibodies that can be used to study the presence of defined proteolytic cleavage sites in aggrecan is described. Principles of peptide design and methods for preparation and characterization of ovalbumin conjugates are presented along with approaches for the characterization and affinity purification of the resulting antisera. Limitations associated with the use of antipeptide antibodies to study authentic protein neoepitopes are discussed.

摘要

本文描述了使用合成肽来生成兔多克隆抗分解代谢新表位抗体，该抗体可用于研究聚集蛋白聚糖中特定蛋白水解切割位点的存在情况。文中介绍了肽设计的原则、卵清蛋白缀合物的制备和表征方法，以及所得抗血清的表征和亲和纯化方法。还讨论了使用抗肽抗体研究真实蛋白质新表位的相关局限性。