Taylor Edward J, Smith Nicola L, Colby John, Charnock Simon J, Black Gary W
School of Health, Natural and Social Sciences, University of Sunderland, Sunderland SR1 3SD, UK.
Antonie Van Leeuwenhoek. 2004 Aug;86(2):167-72. doi: 10.1023/B:ANTO.0000036130.50550.37.
In an attempt to understand better the organisation of genes encoding enzymes of the ribulose monophosphate pathway (RuMP), the 3-hexulose 6-phosphate synthase gene (hps) and flanking sequences were cloned from the obligate methylotroph Aminomonas aminovorus C2A1. To date only three hps containing gene clusters from methylotrophs have been characterised and these contain genes encoding other RuMP enzymes. However, hps from A. aminovorus C2A1 was shown to be adjacent to coding sequences for products with sequence similarity to histidine biosynthesis enzymes. Furthermore, none of the hps homologue containing gene clusters, from genome sequences previously analysed or analysed in this paper, were similar in organisation to that of A. aminovorus C2A1.
为了更好地理解编码磷酸戊糖途径（RuMP）中酶的基因的组织方式，从专性甲基营养菌氨基单胞菌氨基营养菌C2A1中克隆了6-磷酸3-己酮糖合酶基因（hps）及其侧翼序列。迄今为止，仅对来自甲基营养菌的三个含有hps的基因簇进行了表征，这些基因簇包含编码其他RuMP酶的基因。然而，来自氨基单胞菌C2A1的hps被证明与与组氨酸生物合成酶具有序列相似性的产物的编码序列相邻。此外，在先前分析的基因组序列或本文分析的序列中，没有一个含有hps同源物的基因簇与氨基单胞菌C2A1的基因簇在组织上相似。
