[酶联免疫电转移印斑技术在囊尾蚴病免疫诊断中的应用]
[Application of EITB in immunodiagnosis of cysticercosis].
作者信息
Wang Li-na, Ge Ling-yun, Miao Feng, Yu Zhen-hua, Liu Yu-bing, Zhen Tian-min, Li Gui-ping, Yang Shu-fang
机构信息
Department of Immunology, Weifang Medical College, Weifang 261042, China.
出版信息
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2004 Apr 30;22(2):98-100.
OBJECTIVE
To establish a sensitive and specific method EITB for the immunodiagnosis of cysticercosis.
METHODS
Two-dimensional electrophoresis was used to analyze and purify the cyst fluid antigen. Western blotting using the sera of patients with cysticercosis, hydatidosis, and other heteroserum was used to screen the specific antigens. EITB using the specific antigen was established and compared with ELISA. The detection effect of serum and blood on filter paper in EITB was also compared.
RESULTS
Two specific antigens with isoelectric point (pI) of 9.4 and Mr of 14000 and 16600 were obtained, respectively. EITB method based on these antigens was established with the sensitivity and specificity of 92.5% and 100%, respectively, significantly higher than that of ELISA. The sensitivity of serum and filter paper blood was similar in EITB.
CONCLUSION
A sensitive and specific EITB method for immunodiagnosis of cysticercosis was established.
目的
建立一种用于囊尾蚴病免疫诊断的敏感且特异的酶免疫转印印迹法（EITB）。
方法
采用双向电泳分析并纯化囊液抗原。用囊尾蚴病患者血清、包虫病患者血清及其他异源血清进行蛋白质印迹法筛选特异性抗原。建立使用该特异性抗原的EITB法，并与酶联免疫吸附测定（ELISA）法进行比较。同时比较EITB法中血清和滤纸血的检测效果。
结果
分别获得等电点（pI）为9.4、相对分子质量（Mr）为14000和16600的两种特异性抗原。基于这些抗原建立的EITB法敏感性和特异性分别为92.5%和100%，显著高于ELISA法。EITB法中血清和滤纸血的敏感性相似。
结论
建立了一种用于囊尾蚴病免疫诊断的敏感且特异的EITB法。
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