蛋白质印迹法在猪囊尾蚴病诊断中的意义

Significance of western blot in diagnosis of cysticercosis cellulosae.

作者信息

Xu Z B, Liu D

机构信息

Beijing Tropical Medicine Research Institute, WHO Collaborating Center for Paragonimiasis, Clinorchiasis and Leishmaniasis.

出版信息

Chin Med J (Engl). 1992 Dec;105(12):1004-8.

PMID:1299548

Abstract

Sodium dodecyl sulphate (SDS) polyacrylamide gradient gel electrophoresis (PAGE) and immunoblot were used for the diagnosis of patients with cysticercosis cellulosae. 26 KD protein band of the whole Cysticercus antigen showed a high specificity to the sera from patients with cysticercosis cellulosae and had no cross reaction with sera from normal controls and other parasitic diseases including hydatidosis. The positive rate of Western blot with 26 KD band was 70% and the results showed no significant difference in comparison with those of CFT and ELISA.

摘要

采用十二烷基硫酸钠（SDS）聚丙烯酰胺梯度凝胶电泳（PAGE）和免疫印迹法诊断猪囊尾蚴病患者。猪囊尾蚴全抗原的26KD蛋白带对猪囊尾蚴病患者血清具有高度特异性，与正常对照血清及包括包虫病在内的其他寄生虫病血清无交叉反应。以26KD条带进行的免疫印迹法阳性率为70%，与补体结合试验（CFT）和酶联免疫吸附测定（ELISA）结果相比无显著差异。