[细粒棘球绦虫幼虫不同组分特异性抗原的蛋白质免疫印迹分析]

[Western blotting analysis of specific antigens from different components of Echinococcus metacestodes].

作者信息

Jiang Li, Xue Hai-chou, Qiu Li-shu, Feng Zheng

机构信息

Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, WHO Collaborating Centre for Malaria, Schistosomiasis and Filariasis, Shanghai 200025, China.

出版信息

Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2004 Feb 28;22(1):11-5.

PMID:15283257

Abstract

OBJECTIVE

To analyze antigens for searching specific antigenic components for immunodiagnosis of echinococcosis.

METHODS

Fourteen crude antigens from different tissues (cyst fluid, protoscoleces, laminated layer and germinal layer) of Echinococcus granulosus and E. multilocularis metacestodes and other 4 species of cestodes were analyzed by Western blotting. The differences of protein bands were compared for the 14 crude antigens by reacting with pooled sera from cystic echinococcosis (CE) and alveolar echinococcosis (AE) patients.

RESULTS

Eleven protein bands from the antigens reacted nonspecifically with sera from both CE and AE patients were Mr 130000, 100000, 94000, 80000, 75000, 66000, 62000, 52000, 38000, 32000, 24000. The highly specific protein bands recognized by AE sera were Mr 120000, 109000, 86000, 59000, 43000, 28000, 20000, 18000, and by CE sera were Mr 41000, 40000, 22000, 16000 and 12000.

CONCLUSION

Different antigens shared by the two species of Echinococcus were examined and potential antigenic proteins specific for AE or CE sera were found, providing useful information for further identifying specific antigens for immunodiagnosis.

摘要

目的

分析抗原以寻找用于棘球蚴病免疫诊断的特异性抗原成分。

方法

采用蛋白质印迹法分析来自细粒棘球绦虫和多房棘球绦虫成虫不同组织（囊液、原头节、生发层和角质层）的14种粗抗原以及其他4种绦虫的粗抗原。将这14种粗抗原与囊性棘球蚴病（CE）和泡型棘球蚴病（AE）患者的混合血清反应，比较蛋白条带的差异。

结果

14种抗原中的11条蛋白条带与CE和AE患者血清均发生非特异性反应，其相对分子质量分别为130000、100000、94000、80000、75000、66000、62000、52000、38000、32000、24000。AE患者血清识别的高特异性蛋白条带相对分子质量为120000、109000、86000、59000、43000、28000、20000、18000；CE患者血清识别的为41000、40000、22000、16000和12000。