Jiang L, Wen H, Li X, Osman I
Xinjiang Institute for Endemic Diseases Control and Research, National Hydatid Disease Center of China, Urumqi 830002.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1999;17(2):78-80.
To evaluate the 18 kDa antigen in diagnosis of human alveolar echinococcosis.
A total of 214 sera from patients with alveolar echinococcosis (AE, 33), cystic echinococcosis (CE, 69), cysticercosis (30) and healthy controls (82) were examined by Western blotting to detect the antibodies against 18 kDa antigen using both antigens from protoscoleces of E. granulosus (Eg) and E. multilocularis (Em). All the sera were tested simultaneously by ELISA to detect the antibodies against hydatid cystic fluid antigens of sheep as a comparison.
The positive rate by ELISA was 93.9% in AE, 85.5% in CE, 50% in cysticercosis and 6.1% in healthy controls, respectively, suggesting a critical cross-reaction and a certain degree of nonspecific reaction. The serum positive rates by Western blotting with the 18 kDa antigen of Eg and Em were 90.9% and 90.9% in AE, 10.1% and 13% in CE, 13.3% and 16.7% in cysticercosis, respectively. No positive was found in the sera from healthy controls.
The 18 kDa antigen may be used in the differential diagnosis of AE from CE.
评估18 kDa抗原在人体肺泡型棘球蚴病诊断中的价值。
采用Western印迹法，使用细粒棘球绦虫（Eg）和多房棘球绦虫（Em）原头节抗原，检测214份血清，其中肺泡型棘球蚴病（AE，33例）、囊型棘球蚴病（CE，69例）、囊尾蚴病（30例）患者血清及健康对照者（82例）血清中抗18 kDa抗原的抗体。同时采用ELISA法检测所有血清中抗羊棘球蚴囊液抗原的抗体作为对照。
ELISA法检测AE、CE、囊尾蚴病和健康对照者的阳性率分别为93.9%、85.5%、50%和6.1%，提示存在严重交叉反应和一定程度的非特异性反应。用Eg和Em的18 kDa抗原进行Western印迹法检测，AE患者血清阳性率分别为90.9%和90.9%，CE患者分别为10.1%和13%，囊尾蚴病患者分别为13.3%和16.7%。健康对照者血清未发现阳性。
18 kDa抗原可用于AE与CE的鉴别诊断。
