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哈达玛变换毛细管电泳与吸收光谱法联用

Hadamard transform capillary electrophoresis combined with absorption spectrometry.

作者信息

Hata Kazuki, Kaneta Takashi, Imasaka Totaro

机构信息

Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka, 812-8581 Japan.

出版信息

Anal Chem. 2004 Aug 1;76(15):4421-5. doi: 10.1021/ac035366j.

Abstract

A novel injection device for applying absorption spectrometry to Hadamard transform (HT) capillary electrophoresis is described. A small hole, at the center part of the capillary, functions as an inlet port for the sample. The hole is immersed in a sample solution and the end of the capillary that is usually employed for sample introduction is immersed in a buffer solution. An ultraviolet absorption detector is placed between the sample injection port and the other end of the capillary filled with a buffer solution. A high potential is continuously applied between the injection port and the end of the capillary, which allows the sample solution to be introduced into the separation capillary. By application of a higher potential modulated according to a Hadamard code between both ends of the capillary, the buffer solution is injected into the separation capillary. In some preliminary experiments, this injection device was utilized to introduce a single sample segment into a capillary. As expected, a single peak was observed in the electropherogram for a sample containing a single component. This device was then employed for multiple sample injection in HT capillary electrophoresis. An 8-fold improvement in the S/N ratio was observed when the HT technique was used, in which a 255-order of a Hadamard matrix was used, as expected from theory. The present approach was also utilized for the sensitive detection of a sample comprised of multiple components.

摘要

描述了一种用于将吸收光谱法应用于哈达玛变换(HT)毛细管电泳的新型注射装置。毛细管中心部分的一个小孔用作样品的入口。该孔浸入样品溶液中,而通常用于进样的毛细管末端浸入缓冲溶液中。紫外吸收检测器置于进样口和充满缓冲溶液的毛细管另一端之间。在进样口和毛细管末端之间持续施加高电势,这使得样品溶液能够引入到分离毛细管中。通过在毛细管两端之间施加根据哈达玛编码调制的更高电势,缓冲溶液被注入到分离毛细管中。在一些初步实验中,该注射装置被用于将单个样品段引入毛细管。正如预期的那样,对于含有单一成分的样品,在电泳图中观察到一个单峰。然后该装置被用于HT毛细管电泳中的多次进样。当使用HT技术(其中使用255阶的哈达玛矩阵)时,信噪比提高了8倍,这与理论预期一致。本方法还用于对由多种成分组成的样品进行灵敏检测。

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