使用聚合酶链反应（PCR）对新鲜粪便样本中的致病性溶组织内阿米巴进行选择性鉴定。

Selective identification of the pathogenic E. histolytica in fresh stool samples using polymerase chain reaction (PCR).

作者信息

el-Hamshary Eman M, el-Shewy Khalid A, Hezagy Mamdouh M, Zakaria Hanan

机构信息

Department of Parasitology, Faculties of Medicine, Suez Canal University, Egypt.

出版信息

J Egypt Soc Parasitol. 2004 Aug;34(2):611-20.

PMID:15287183

Abstract

Stool samples from 93 individuals clinically presumed to have intestinal amoebiasis and subjected to microscopic examination and DNA extraction. The PCR amplification was performed using two sets of primers that differentiate between pathogenic and nonpathogenic Entamoeba DNA. Of 93 clinically positive cases, 51 (54.8%) were positive by microscopy, while 53 (56.9%) were detected by PCR as having DNA specific for either E. histolytica / E. dispar. A specificity of 85.71% and a sensitivity of 92.15% were with PCR compared to microscopy. Among 53 PCR positive specimens, three different DNA sequences were demonstrated: 8 specimens had DNA sequences specific of E. histolytica, 31 with DNA specific for E. dispar and 14 specimens have mixed DNA sequences for E. histolytica and E. dispar. PCR is a sensitive and a specific tool. PCR application is better the epidemiology in endemic areas through keeping indefinite DNA records for prospective and retrospective studies.

摘要

从93名临床上疑似患有肠道阿米巴病的个体采集粪便样本，并进行显微镜检查和DNA提取。使用两组引物进行PCR扩增，以区分致病性和非致病性溶组织内阿米巴DNA。在93例临床阳性病例中，51例（54.8%）显微镜检查呈阳性，而53例（56.9%）通过PCR检测出具有溶组织内阿米巴/迪氏内阿米巴特异性DNA。与显微镜检查相比，PCR的特异性为85.71%，敏感性为92.15%。在53例PCR阳性标本中，显示出三种不同的DNA序列：8例具有溶组织内阿米巴特异性DNA序列，31例具有迪氏内阿米巴特异性DNA，14例标本具有溶组织内阿米巴和迪氏内阿米巴的混合DNA序列。PCR是一种敏感且特异的工具。通过保存无限期的DNA记录用于前瞻性和回顾性研究，PCR的应用有助于更好地了解流行地区的流行病学情况。