澳大利亚悉尼粪便样本中溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维茨内阿米巴的聚合酶链反应检测
PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia.
作者信息
Fotedar R, Stark D, Beebe N, Marriott D, Ellis J, Harkness J
机构信息
Department of Microbiology, St. Vincent's Hospital, Sydney, Australia.
出版信息
J Clin Microbiol. 2007 Mar;45(3):1035-7. doi: 10.1128/JCM.02144-06. Epub 2007 Jan 17.
Abstract
This study investigated the presence of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from a patient population in Sydney, Australia. Stool samples were tested by microscopy and PCR. Five patients were found with E. histolytica infections, while E. dispar and E. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by PCR. This is the first study in Australia using molecular techniques to determine the presence of E. histolytica, E. dispar, and E. moshkovskii.
摘要
本研究调查了澳大利亚悉尼患者群体粪便样本中溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维茨内阿米巴的存在情况。粪便样本通过显微镜检查和聚合酶链反应（PCR）进行检测。发现5例患者感染了溶组织内阿米巴，而通过PCR分别在63例（70.8%）和55例（61.8%）患者中观察到了迪斯帕内阿米巴和莫斯科维茨内阿米巴。这是澳大利亚首次使用分子技术来确定溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维茨内阿米巴的存在情况的研究。
相似文献
1
PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia.澳大利亚悉尼粪便样本中溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维茨内阿米巴的聚合酶链反应检测
J Clin Microbiol. 2007 Mar;45(3):1035-7. doi: 10.1128/JCM.02144-06. Epub 2007 Jan 17.
2
Entamoeba species associated with chronic diarrhoea in Pakistan.与巴基斯坦慢性腹泻相关的内阿米巴物种。
Epidemiol Infect. 2012 Feb;140(2):323-8. doi: 10.1017/S0950268811000215. Epub 2011 Mar 14.
3
Differential detection of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii in fecal samples by nested PCR in the United Arab Emirates (UAE).在阿拉伯联合酋长国（阿联酋）通过巢式聚合酶链反应（nested PCR）对粪便样本中溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维茨内阿米巴进行鉴别检测 。
Acta Parasitol. 2013 Jun;58(2):185-90. doi: 10.2478/s11686-013-0128-8. Epub 2013 May 11.
4
A novel nested multiplex polymerase chain reaction (PCR) assay for differential detection of Entamoeba histolytica, E. moshkovskii and E. dispar DNA in stool samples.一种用于粪便样本中溶组织内阿米巴、摩西阿米巴和迪斯帕内阿米巴DNA差异检测的新型巢式多重聚合酶链反应（PCR）检测方法。
BMC Microbiol. 2007 May 24;7:47. doi: 10.1186/1471-2180-7-47.
5
6
PCR differentiation of Entamoeba histolytica and Entamoeba dispar from patients with amoeba infection initially diagnosed by microscopy.通过聚合酶链反应（PCR）对最初经显微镜检查诊断为阿米巴感染患者的溶组织内阿米巴和迪斯帕内阿米巴进行鉴别。
Scand J Infect Dis. 2005;37(9):680-5. doi: 10.1080/00365540510037812.
7
Entamoeba histolytica and Entamoeba dispar: prevalence infection in a rural Mexican community.溶组织内阿米巴和迪氏内阿米巴：墨西哥农村社区的感染率
Exp Parasitol. 2005 Jul;110(3):327-30. doi: 10.1016/j.exppara.2005.03.015. Epub 2005 Apr 20.
8
Differential detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii by a single-round PCR assay.通过单轮聚合酶链反应检测法对溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维奇内阿米巴进行鉴别检测
J Clin Microbiol. 2006 Sep;44(9):3196-200. doi: 10.1128/JCM.00778-06.
9
10
Entamoeba moshkovskii and Entamoeba dispar-associated infections in pondicherry, India.印度本地治里地区与摩西内阿米巴和迪斯帕内阿米巴相关的感染
J Health Popul Nutr. 2005 Sep;23(3):292-5.
引用本文的文献
1
Identification of Species in Diarrheal Samples Using Sequence Analysis and Nested Multiplex PCR.利用序列分析和巢式多重聚合酶链反应鉴定腹泻样本中的物种
Iran J Parasitol. 2025 Apr-Jun;20(2):253-260. doi: 10.18502/ijpa.v20i2.19032.
2
A New Multiplex PCR Assay Reveals the Occurrence of E. bangladeshi alongside E. histolytica and E. moshkovskii in Eastern India.一种新的多重PCR检测方法揭示了在印度东部，孟加拉埃希氏菌与溶组织内阿米巴和莫斯科维奇埃希氏菌共同存在的情况。
Acta Parasitol. 2024 Dec;69(4):1886-1895. doi: 10.1007/s11686-024-00921-z. Epub 2024 Oct 10.
3
Genetic characterization of the population based on different potential genetic markers.基于不同潜在遗传标记对人群进行基因特征分析。
Parasitology. 2024 Apr;151(4):429-439. doi: 10.1017/S003118202400026X. Epub 2024 Mar 11.
4
Development of nucleic acid lateral flow immunoassay for molecular detection of Entamoeba moshkovskii and Entamoeba dispar in stool samples.开发用于粪便样本中莫氏内阿米巴和迪斯帕内阿米巴分子检测的核酸侧向流动免疫分析。
Sci Rep. 2024 Mar 19;14(1):6635. doi: 10.1038/s41598-024-57332-3.
5
VESPA: an optimized protocol for accurate metabarcoding-based characterization of vertebrate eukaryotic endosymbiont and parasite assemblages.VESPA：一种优化的基于宏条形码的脊椎动物真核内共生体和寄生虫类群特征描述的方案。
Nat Commun. 2024 Jan 9;15(1):402. doi: 10.1038/s41467-023-44521-3.
6
Profile of Amoebic vs Pyogenic Liver Abscess and Comparison of Demographical, Clinical, and Laboratory Profiles of these Patients From a Tertiary Care Center in Northern India.印度北部一家三级医疗中心的阿米巴性与化脓性肝脓肿病例概况及这些患者的人口统计学、临床和实验室特征比较
J Clin Exp Hepatol. 2023 Nov-Dec;13(6):1025-1031. doi: 10.1016/j.jceh.2023.07.002. Epub 2023 Jul 7.
7
Amoebiasis: Advances in Diagnosis, Treatment, Immunology Features and the Interaction with the Intestinal Ecosystem.阿米巴病：诊断、治疗、免疫学特征的进展及与肠道生态系统的相互作用。
Int J Mol Sci. 2023 Jul 21;24(14):11755. doi: 10.3390/ijms241411755.
8
Frequency of Complex in Individuals Referred to the Medical Laboratories in Jahrom City, South of Iran.伊朗南部贾赫罗姆市医学实验室送检个体中复合体的频率
J Parasitol Res. 2023 Jul 10;2023:8914563. doi: 10.1155/2023/8914563. eCollection 2023.
9
Intestinal Parasitic Infections in 2023.2023年的肠道寄生虫感染
Gastroenterology Res. 2023 Jun;16(3):127-140. doi: 10.14740/gr1622. Epub 2023 Jun 11.
10
Molecular-Genotyping Detection of in Diarrheic Patients.腹泻患者中 的分子基因分型检测
Arch Razi Inst. 2023 Feb 28;78(1):337-343. doi: 10.22092/ARI.2022.358947.2336. eCollection 2023 Feb.
本文引用的文献
1
Differential detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii by a single-round PCR assay.通过单轮聚合酶链反应检测法对溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维奇内阿米巴进行鉴别检测
J Clin Microbiol. 2006 Sep;44(9):3196-200. doi: 10.1128/JCM.00778-06.
2
Entamoeba moshkovskii and Entamoeba dispar-associated infections in pondicherry, India.印度本地治里地区与摩西内阿米巴和迪斯帕内阿米巴相关的感染
J Health Popul Nutr. 2005 Sep;23(3):292-5.
3
PCR differentiation of Entamoeba histolytica and Entamoeba dispar from patients with amoeba infection initially diagnosed by microscopy.通过聚合酶链反应（PCR）对最初经显微镜检查诊断为阿米巴感染患者的溶组织内阿米巴和迪斯帕内阿米巴进行鉴别。
Scand J Infect Dis. 2005;37(9):680-5. doi: 10.1080/00365540510037812.
4
Real-time-PCR assay for diagnosis of Entamoeba histolytica infection.用于诊断溶组织内阿米巴感染的实时荧光定量聚合酶链反应检测法
J Clin Microbiol. 2005 May;43(5):2168-72. doi: 10.1128/JCM.43.5.2168-2172.2005.
5
The use of real-time PCR to identify Entamoeba histolytica and E. dispar infections in prisoners and primary-school children in Ethiopia.利用实时聚合酶链反应鉴定埃塞俄比亚囚犯和小学生中的溶组织内阿米巴和迪斯帕内阿米巴感染情况。
Ann Trop Med Parasitol. 2004 Jan;98(1):43-8. doi: 10.1179/000349804225003082.
6
Present characteristics of symptomatic Entamoeba histolytica infection in the big cities of Japan.日本大城市中症状性溶组织内阿米巴感染的当前特征。
Epidemiol Infect. 2004 Jan;132(1):57-60. doi: 10.1017/s0950268803001389.
7
Entamoeba moshkovskii infections in children, Bangladesh.孟加拉国儿童中的莫氏内阿米巴感染
Emerg Infect Dis. 2003 May;9(5):580-4. doi: 10.3201/eid0905.020548.
8
Estimating the impact of amebiasis on health.评估阿米巴病对健康的影响。
Parasitol Today. 2000 Aug;16(8):320-1. doi: 10.1016/s0169-4758(00)01730-0.
9
Simple differential detection of Entamoeba histolytica and Entamoeba dispar in fresh stool specimens by sodium acetate-acetic acid-formalin concentration and PCR.通过乙酸钠-乙酸-甲醛浓缩法和聚合酶链反应对新鲜粪便标本中的溶组织内阿米巴和迪斯帕内阿米巴进行简易鉴别检测。
J Clin Microbiol. 1997 Jul;35(7):1701-5. doi: 10.1128/jcm.35.7.1701-1705.1997.
10
A redescription of Entamoeba histolytica Schaudinn, 1903 (Emended Walker, 1911) separating it from Entamoeba dispar Brumpt, 1925.对溶组织内阿米巴（1903年，绍丁命名，1911年沃克修订）的重新描述，将其与1925年布伦普特命名的迪斯帕内阿米巴区分开来。
J Eukaryot Microbiol. 1993 May-Jun;40(3):340-4. doi: 10.1111/j.1550-7408.1993.tb04926.x.
Zotero 插件