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澳大利亚悉尼粪便样本中溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维茨内阿米巴的聚合酶链反应检测

PCR detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from Sydney, Australia.

作者信息

Fotedar R, Stark D, Beebe N, Marriott D, Ellis J, Harkness J

机构信息

Department of Microbiology, St. Vincent's Hospital, Sydney, Australia.

出版信息

J Clin Microbiol. 2007 Mar;45(3):1035-7. doi: 10.1128/JCM.02144-06. Epub 2007 Jan 17.

DOI:10.1128/JCM.02144-06
PMID:17229864
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1829108/
Abstract

This study investigated the presence of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in stool samples from a patient population in Sydney, Australia. Stool samples were tested by microscopy and PCR. Five patients were found with E. histolytica infections, while E. dispar and E. moshkovskii were observed in 63 (70.8%) and 55 (61.8%) patients, respectively, by PCR. This is the first study in Australia using molecular techniques to determine the presence of E. histolytica, E. dispar, and E. moshkovskii.

摘要

本研究调查了澳大利亚悉尼患者群体粪便样本中溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维茨内阿米巴的存在情况。粪便样本通过显微镜检查和聚合酶链反应(PCR)进行检测。发现5例患者感染了溶组织内阿米巴,而通过PCR分别在63例(70.8%)和55例(61.8%)患者中观察到了迪斯帕内阿米巴和莫斯科维茨内阿米巴。这是澳大利亚首次使用分子技术来确定溶组织内阿米巴、迪斯帕内阿米巴和莫斯科维茨内阿米巴的存在情况的研究。

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Real-time-PCR assay for diagnosis of Entamoeba histolytica infection.用于诊断溶组织内阿米巴感染的实时荧光定量聚合酶链反应检测法
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