通过随机扩增多态性DNA（RAPD）和核糖体分型对来自津巴布韦猪萎缩性鼻炎病例的多杀性巴氏杆菌分离株进行鉴别

Differentiation of Pasteurella multocida isolates from cases of atrophic rhinitis in pigs from Zimbabwe by RAPD and ribotyping.

作者信息

Dziva F, Christensen H, van Leengoed L A M G, Mohan K, Olsen J E

机构信息

Faculty of Veterinary Science, University of Zimbabwe, PO Box MP167, Mount Pleasant, Harare.

出版信息

Vet Microbiol. 2004 Aug 19;102(1-2):117-22. doi: 10.1016/j.vetmic.2004.06.006.

DOI:10.1016/j.vetmic.2004.06.006

PMID:15288934

Abstract

Atrophic rhinitis in pigs is rarely reported in Southern Africa. To determine the relationship between Pasteurella multocida clones from clinical cases of atrophic rhinitis, twenty-one strains were characterised by selected phenotypic and genotypic methods. Biochemical analysis classified 18 strains as P. multocida subspecies multocida, whilst the remainder were grouped into separate unassigned biotypes. Capsular groups A (16/21) and D (l/21) were found among the isolates by PCR. Four ribotype patterns were obtained following HpaII ribotyping, whilst random amplification of polymorphic DNA (RAPD) revealed three main clusters. However, subclusters were also noted for each RAPD cluster. Our results indicate that RAPD offers a better discrimination of strains than ribotyping and that none of the phenotypic characters were directly related to the genotypic clusters.

摘要

在南部非洲，猪萎缩性鼻炎鲜有报道。为确定来自萎缩性鼻炎临床病例的多杀性巴氏杆菌克隆之间的关系，采用选定的表型和基因型方法对21株菌株进行了鉴定。生化分析将18株菌株归类为多杀性巴氏杆菌多杀亚种，其余菌株则被归入未分类的不同生物型。通过PCR在分离株中发现了A群（16/21）和D群（1/21）荚膜。经HpaII核糖分型获得了四种核糖型模式，而随机扩增多态性DNA（RAPD）揭示了三个主要聚类。然而，每个RAPD聚类也有亚聚类。我们的结果表明，与核糖分型相比，RAPD对菌株的鉴别能力更强，且没有任何表型特征与基因型聚类直接相关。